The article presents an analysis of the literature on currently available methods for diagnosing acute intestinal infection (Oki) of viral etiology. This work considers the ELISA method, identifies it’s advantages and disadvantages compared to other known methods of laboratory diagnostics. The effectiveness of this method was confirmed by the study of changes in the concentration of viral antigen in fecal samples of patients aged 18 to 85 with rot and norovirus infection. Carrying out ELISA with use of a set of reagents vector-best allowed to establish the concentration of viral antigens in the copromaterial of patients, as well as to determine the severity of the disease in individuals of different ages and genders. It was found that rotavirus infection was acute and severe among women of age 36-55 and with an average degree of severity among men of age 36-60. During the norovirus infection, short-term symptoms of gastroenteritis accompanied by moderate or light pain and intoxication syndrome, are most characteristic.
acute intestinal infection, enzyme-linked immunosorbent assay, norovirus, rotavirus
1. Baranov A.A. Detskie bolezni: uchebnik. Moskva: GEOTAR-MED, 2002, 880 s. @@[Baranov A.A. Children's diseases: textbook. Moscow: GEOTAR-MED, 2002, 880 p. (In Russ.)]
2. Bulatov V.P., Kamalova A.A. Hronicheskaya diareya u detey. Prakticheskaya medicina, 2012, № 7, s. 38-40. @@[Bulatov V.P., Kamalova A.A. Chronic diarrhea in children. Prakticheskaya medicina, 2012, no. 7, pp. 38-40. (In Russ.)]
3. Grigor'ev P.Ya., Yakovenko A.V. Klinicheskaya gastroenterologiya: uchebnik. Moskva: Medicinskoe informacionnoe agentstvo, 2004, 768 s. @@[Grigoriev P.Ya., Yakovenko A.V. Clinical gastroenterology: textbook. Moscow: Medicinskoe informacionnoe agentstvo, 2004, 768 p. (In Russ.)]
4. Guteneva I.V. Razrabotka metodov immunohromatograficheskoy detekcii malyh molekul s ispol'zovaniem magnitnyh nanomarkerov: special'nost' 03.01.02 «Biofizika»: diss. … kand. fiz.-mat. nauk / Guteneva Nataliya Viktorovna: Moskovskiy fiziko-tehnicheskiy institut (nacional'nyy issledovatel'skiy universitet). Moskva, 2019, 119 s. @@[Guteneva I. V. Development of methods for immunochromatographic detection of small molecules using magnetic nanomarkers: specialty 03.01.02 "Biophysics": dissertation … candidate of physical and mathematical Sciences / Natalia Guteneva: Moskovskij fiziko-tekhnicheskij institut (nacional'nyj issledovatel'skij universitet). Moscow, 2019, 119 p. (In Russ.)]
5. Instrukciya po primeneniyu nabora reagentov dlya IFA Vektor-Best. Rezhim dostupa: http://art-medika.com/catalog/IFA/infection/virusy (data obrascheniya 06.07.2020). @@[Instructions for using a set of reagents for ELISA Vector-best. Access mode: http://art-medika.com/catalog/IFA/infection/virusy (date of request: 06.07.2020) (In Russ.)]
6. Hohlova N.I., Kapustin D.V., Krasnova E.I., Izvekova I.Ya. Norovirusnaya infekciya (obzor literatury). Zhurnal infektologii, 2018, № 10, s. 5-14. @@[Khokhlova N.I., Kapustin D.V., Krasnova E.I., Izvekova I.Ya. Norovirus infection (systematic review). ZHurnal infektologii, 2018, no. 10, pp. 5-14. DOI:https://doi.org/10.22625/2072-6732-2018-10-1-5-14. (In Russ.)]
7. O sostoyanii sanitarno-epidemiologicheskogo blagopoluchiya naseleniya v Rossiyskoy Federacii v 2018 godu: Gosudarstvennyy doklad. Moskva: Federal'naya sluzhba po nadzoru v sfere zaschity prav potrebiteley i blagopoluchiya cheloveka, 2019, 254 s. @@[On the state of sanitary and epidemiological welfare of the population in the Russian Federation in 2018: State report. Moscow: Federal'naya sluzhba po nadzoru v sfere zashchity prav potrebitelej i blagopoluchiya cheloveka, 2019, 254 p. (In Russ.)]
8. Zhirakovskaya E.V., Ignat'ev G.M., Indikova I.N., Tikunova N.V. Ocenka novoy IFA test-sistemy «Rotavirus-antigen-IFA-BEST». BIOpreparaty. Profilaktika, diagnostika, lechenie, 2009, № 3-4, s. 23-38. @@[Jirkovsky E.V., Ignatiev G.M., Indikov I.N., Tikunova N.V. Evaluation of the new ELISA test system «Rotavirus-antigen-ELISA-BEST» BIOpreparaty. Profilaktika, diagnostika, lechenie, 2009, № 3-4, pp. 23-38. (In Russ.)]
9. Sagalova O.I. Kliniko-immunologicheskaya harakteristika kishechnyh infekciy virusnoy etiologii u vzroslyh: special'nost' 14.00.10: avtoreferat diss. … d-a med. nauk / Sagalova Ol'ga Igorevna: Central'nyy nauchno-issledovatel'skiy institut epidemiologii MZ RF. Moskva, 2009, 42 s. @@[Sagalova O.I. Clinical and immunological characteristics of intestinal infections of viral etiology in adults: specialty 14.00.10: abstract of the dissertation … of the doctor of medical Sciences / Sagalova Olga Igorevna: Central'nyj nauchno-issledovatel'skij institut epidemiologii MZ RF. Moscow, 2009, 42 p. (In Russ.)]
10. Epidemiologicheskiy nadzor, laboratornaya diagnostika i profilaktika norovirusnoy infekcii: 3.1.1 Profilaktika infekcionnyh bolezney. Kishechnye infekcii: metodicheskie ukazaniya: MU 3.1.1.2969-11 / Federal'naya sluzhba po nadzoru v sfere zaschity prav potrebiteley i blagopoluchiya cheloveka. Moskva: Rospotrebnadzor: Federal'nyy centr gigieny i epidemiologii Rospotrebnadzora, 2012, 34 s. @@[Epidemiological surveillance, laboratory diagnostics and prevention of norovirus infection: 3.1.1 prevention of infectious diseases. Intestinal infections: guidelines: MU 3.1.1.2969-11 / Federal service for supervision of consumer rights protection and human welfare. - Moscow: Rospotrebnadzor: Federal'nyj centr gigieny i epidemiologii Rospotrebnadzora, 2012, 34 p. (In Russ.)]
