Moscow, Moscow, Russian Federation
The sensitivity of cultured mesenchymal stromal stem cells (MSC) obtained from mouse bone marrow to irradiation with 32 MeV protons and the relative biological efficiency (RBE) of protons in the low dose range were studied. The survival of MSCs after irradiation was assessed by the change in the number of cells and in their clonogenic activity. Dosimetry of proton radiation was performed using an EBT3 radiochromic film, which was irradiated under the same conditions as the irradiated cells. For this, the film samples were placed in similar cylindrical tubes in the culture medium, simulating the conditions for irradiation of the cell suspension. The distribution of the energy release of protons during the passage of a particle beam through the samples under study was calculated using the SRIM-2013-Pro software. It has been shown that both the total number of MSCs and the number of MSCs capable of proliferation decrease with an increase in the proton irradiation dose. Moreover, cells with clonogenic activity are more sensitive to inactivation by protons than the general population of irradiated MSCs. When comparing the sensitivity of the clonogenic activity of MSCs to irradiation with protons and gamma rays, it was shown that cells in the range of low doses (from 0.15 to 0.6 Gy) are more sensitive to the action of protons. When calculating the RBE of protons from the data obtained, an increase in this indicator was shown in the indicated dose range from 1.54 to 2.07.
accelerated particles, protons, linear energy transfer, dosimetry, dosimetry film EBT3, RBE, relative biological effectiveness, gamma radiation, mesenchymal stem cells, clonogenic activity
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