It has been established that low-temperature luminous bacteria have specific emission and spectral characteristics. In vivo, all isolated strains produce intense bioluminescence (up to 105 quant/s. per cell) with luminescence maxima of about 478-480 nm with shoulders at 500-510 and 530-540 nm. The spectra were decomposed at different temperatures. The total spectrum is well decomposed into 3 components corresponding to luciferase, BFP and YFP emitters. The analysis of the temperature dependence of cell bioluminescence at wavelengths corresponding to the emission maxima of these components in the range of activation (4-200С) and decay (22-320С). The bioluminescence spectrum is interpreted by the presence in low-temperature photobacteria species the auxiliary emitters, which, in combination with luciferase, participate in the bioluminescence process.
photobacteria, bioluminescence, Photobacterium phosphoreum
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