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Home / Journals / Russian Journal of Biological Physics and Chemisrty / Volume 6 Issue 1 / Study of the technology of the recombinate target protein selective labeling and development of the synthetic routes for the modified CdTe quantum dots synthesis in aqueous solutions
Study of the technology of the recombinate target protein selective labeling and development of the synthetic routes for the modified CdTe quantum dots synthesis in aqueous solutions
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STUDY OF THE TECHNOLOGY OF THE RECOMBINATE TARGET PROTEIN SELECTIVE LABELING AND DEVELOPMENT OF THE SYNTHETIC ROUTES FOR THE MODIFIED CDTE QUANTUM DOTS SYNTHESIS IN AQUEOUS SOLUTIONS
UDK 577 Материальные основы жизни. Биохимия. Молекулярная биология.Биофизика
Belikov N E 1
Demina O V 2
Lukin A Yu 3
Petrovskaya L E 4
Skladnev D A 5
Varfolomeev S D 6
Khodonov A A 7
Authors:
1.  N.M. Emanuel Institute of Biochemical Physics, RAS

2.  N.M. Emanuel Institute of Biochemical Physics, RAS

3.  MIREA - Russian Technological University

4.  M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, RAS

5.  Federal Research Centre «Fundamentals of Biotechnology», RAS

6.  N.M. Emanuel Institute of Biochemical Physics, RAS

7.  N.M. Emanuel Institute of Biochemical Physics, RAS

Type:
Article
Pages:
from 88 to 96
Status:
Published
Received:
20.03.2021
Accepted:
20.03.2021
Published:
25.03.2021
Subject area:
UDK 577 Материальные основы жизни. Биохимия. Молекулярная биология.Биофизика
Language:
Russian
Keywords:
selective labeling of target proteins, fluorescent labels, quantum dots, production methods, nitrilotriacetic acid
Funding:
Issledovanie vypolneno pri finansovoy podderzhke RFFI v ramkah nauchnogo proekta № 20-03-00139.
Abstract and keywords
Abstract (English):
This paper considers the proposed procedure for reversible labeling of target proteins using fluorescent labels and probes. New fluorophores based on quantum dots (QDs CdTe) containing a molecular address (chelate complexes of Ni2+ with nitrilotriacetic acid and its derivatives) were proposed, capable of forming strong chelates with a hexahistidine tag localized at a given site of the polypeptide chain of the target protein. In this work, special attention is devoted to the selection of the required composition and structure of the label molecule and the practical implementation of the laboratory technology for the synthesis of a set of initial QDs CdTe quantum dots samples with specified optical parameters.

Keywords:
selective labeling of target proteins, fluorescent labels, quantum dots, production methods, nitrilotriacetic acid
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References

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