Kotelnikov Institute of Radioengineering and Electronics of RAS (Fryazino branch)
Moscow, Moscow, Russian Federation
Pirogov Russian National Research Medical University
Kotelnikov Institute of Radioengineering and Electronics of RAS (Fryazino branch)
Moscow, Moscow, Russian Federation
Kotelnikov Institute of Radioengineering and Electronics of RAS (Fryazino branch)
Moscow, Moscow, Russian Federation
Free radical reactions play an important role in protecting the body from infections, in particular, in the response of cells of the immune system - neutrophils and macrophages to pathogens. The results of our studies showed that the response of neutrophils to various activators was enhanced as a result of exposure to microwave radiation. Irradiation changed the chemiluminescence (CL) kinetics of whole blood, to which activators were added, so that the CL of the irradiated blood samples was significantly higher. Comparison of the results of heating blood samples under the influence of microwave radiation and in a thermostat showed the same increase in the CL response of activated neutrophils. Measurement of CL of isolated neutrophils resuspended in plasma proved that the enhancement of neutrophil activation during irradiation is not mediated by other blood cells or platelets. Morphological analysis showed that microwave radiation activates neutrophils, i.e. increases the percentage of activated cells in suspension. It was also shown that radiation can weakly but reliably enhance the functional response of myeloperoxidase, an important neutrophil protein that is capable of producing reactive oxygen species (ROS). Thus, our studies have shown that microwave radiation enhances the response of neutrophils to the activation inducer in whole blood, increasing the production of ROS in the blood.
microwave radiation, human blood neutrophils, oxidative stress, chemiluminescence, reactive oxygen species, myeloperoxidase, EHF range
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