Baku, Azerbaijan
Baku, Azerbaijan
In experiments on yeast cells, the effect of various doses (5-100 Gу) of γ-rays was studied. Respiration sensitivity and rate of superoxide radical formation were investigated in irradiated yeast cells. The study of the parameters of LucCL in irradiated yeast cells revealed an increase in the reaction of ROS formation compared to control cells , which indicates an increase in the rate of formation of the superoxide anion radical (•O2-) and, thus, a high activity of NADPH-oxidase. Polarographic curves of oxygen absorption by C. guilliermondii cells show that as a result of irradiation of cells by low doses (5-50 Gу), was watched a slight inhibition of cell respiration. The study of the effect of high doses (75-100 Gу) showed that they significantly inhibit the respiration of yeast cells. These data coincide with the data when studying the effect of these doses on changes in the physical state of membranes and the survival of yeast cells. The obtained data allow us to conclude that there are differences in the response of yeast cells to the action of low and high doses of gamma radiation.
ionizing radiation, γ-rays, yeast cells, ROS, superoxide radical
1. Zyuzikov N.A., Korogodin V.I., Korogodina V.L. Osobennosti deystviya malyh doz γ -izlucheniya na drozhzhevye kletki. Radiacionnaya biologiya. Radioekologiya, 1999, t. 39, № 6, c. 619-622. @@Zyuzikov N.A., Korogodin V.I., Korogodina V.L. Features of the action of small doses of γ-radiation on yeast cells. Radiation Biology. Radioecology, 1999, vol. 39, no. 6, pp. 619-622. (In Russ.)]
2. Komarova L.N. Proyavlenie adaptivnoy reakcii u drozhzhevyh kletok posle deystviya ioniziruyuschey radiacii. Mediko-biologicheskie problemy zhiznedeyatel'nosti, 2015, № 2 (14). @@Komarova L.N. Manifestation of an adaptive response in yeast cells after exposure to ionizing radiation. Medical and biological problems of life, 2015, no. 2 (14). (In Russ.)]
3. Kocharli, N.K, Gummatova S.T. Ctrukturno-funkcional'noe sostoyanie plazmaticheskih membran kletok drozhzhey pri deystvii γ - izlucheniya. Aktual'nye voprosy biologicheskoy fiziki i himii, 2020, t. 5, № 2, s. 223-229. @@Kocharli N.K., Gummatova S.T. Structural and functional state of plasma membranes of yeast cells under the action of γ-radiation.Russian Journal of Biological Physics and Chemistry, 2020, vol. 5, no. 2, pp. 223-229. (In Russ.)]
4. Dutta K., Verma N. Exposure to low dose of gamma radiation enhances the excision repair in Saccharomyces cerevisiae. J. Gen. Appl. Microbiol., 1998, vol. 44, pp. 243-249.
5. Wolff S. et al. Human lymphocytes exposed to low doses of ionizing radiation become refractory to high doses of radiation as well as to chemical mutagens that induce doublestrand breaks in DNA.Int. J. Radiat. Biol., 1988, vol. 53.
6. Ritter G.S., Nikolin V.P. et al. Characteristic of the active substance of the Saccharomyces cerevisiae preparation having radioprotective properties.Int. J. Radiat. Biol., 2020, vol. 96, no. 9, pp. 1173-1191. doi:https://doi.org/10.1080/09553002.2020.1793020.
7. Akaboshi E., Howard-Flanders P. Proteins induced by DNA-damaging agents in cultured Drosophila cells. Mutat. Res., 1989, vol. 227, pp. 1-6.
8. Iriskulov B.U. Modern condition of the problem of lipid peroxide oxidation. Central Asian Journal of Medicine, vol. 2019, iss. 1, article 7.
9. Jiapeng Z., Kutti R. Vinothkumar, Judy Hirst. Structure of mammalian respiratory complex I. Nature, 2016, vol. 536, pp. 80-84.
10. Vladimirov Yu.A., Proskurnina E.V. Svobodnye radikaly i Kletochnaya Hemilyuminescenciya. Uspehi biologicheskoy himii, 2009, t. 49, s. 341-388. @@Vladimirov Yu.A., Proskurnina E.V. Free Radicals and Cellular Chemiluminescence. Advances in Biological Chemistry, 2009, vol. 49, pp. 341-388 (In Russ.)]
11. Obrazcov I.V., Godkov M.A. Xemilyuminescentnyy analiz kletok krovi v medicine: istoriya, teoriya, praktika. Federal'nyy nauchno-klinicheskiy centr detskoy gematologii, onkologii i immunologii. M., 2013, s. 6-7. @@Obraztsov I.V., Godkov M.A. Chemiluminescent analysis of blood cells in medicine: history, theory, practice. Federal Scientific Clinical Center of Pediatric Hematology, Oncology and Immunology. Moscow, 2013, pp. 6-7. (In Russ.)]
12. Beloborodova N. et al. Effect of phenolic acids of microbial origin on production ofreactive oxygen species in mitochondria and neutrophils. J. Biomed. Sci., 2012, vol. 19, no. 89.
13. Novikov V.E. Issledovanie kinetiki dyhaniya drozhzhevyh kletok polyarograficheskim metodom. Praktikum biofizika kletki, 2001, s. 5-7. @@Novikov V.E. Investigation of the kinetics of respiration of yeast cells by the polarographic method. Practical biophysics of cells, 2001, pp. 5-7. (In Russ.)]
14. Vladimirov Yu.A. Fluorescentnye zondy v issledovanii biologicheskih membran, 1980, 350 c. @@Vladimirov Yu.A. Fluorescent probes in the study of biological membranes, 1980, 350 p. (In Russ.)]
