DECOMPOSITION NATIVE ABSORPTION SPECTRUM OF THE CULTURE MICRO ALGA ARTHROSPIRA (SPIRULINA) PLATENSIS
Abstract and keywords
Abstract (English):
The work carried out a mathematical separation of the absorption spectrum of the culture of the microalga Spirulina platensis. Culture spectra were recorded on an integrating sphere spectrophotometer. When analyzing the spectrum, the second-order derivative was calculated and the main absorption maxima were determined. The obtained data served as the basis for modeling. The absorption spectrum of the culture of the microalga Spirulina platensis in the region from 400 to 800 nm was described by six Gaussian curves. A general model of the absorption spectrum of a culture from individual pigments was compiled. Using the resulting model, it is possible to determine the concentration of chlorophyll a, total carotenoids and phycobilins directly from the absorption spectrum of the native culture.

Keywords:
Spirulina, carotenoids, chlorophyll a, phycobilins, absorption spectra, Gaussian curves
Text
Publication text (PDF): Read Download
References

1. Kopytov Yu.P. i dr. Metodika kompleksnogo opredeleniya biohimicheskogo sostava mikrovodorosley. Al'gologiya, 2015, t. 25, № 1, s. 35-40. @@Kopytov Yu.P. et al. The method of complex determination of the biochemical composition of microalgae. Algologiya, 2015, vol. 25, no. 1, c. 35-40. (In Russ.)]

2. Gevorgiz R.G., Nehoroshev M.V. Kolichestvennoe opredelenie massovoy doli S-fikocianina i allofikocianina v suhoy biomasse Spirulina (Arthrospira) platensis North. Geitl. Holodnaya ekstrakciya: uchebno-metodicheskoe posobie. RAN, In-t morskih biologicheskih issledovaniy im. A.O. Kovalevskogo. Sevastopol', 2017, 21 s. (Preprint RAN, IMBI). @@Gevorgiz R.G., Nekhoroshev M.V. Quantitative determination of the mass fraction of C-phycocyanin and allophycocyanin in the dry biomass of Spirulina (Arthrospira) platensis North. Geitl. Cold extraction: an educational and methodological manual. RAS, Institute of Marine Biological Research named after A.O. Kovalevsky. Sevastopol, 2017, 21 p. (Preprint RAS, IMBI). (In Russ.)]

3. Wellburn R.W. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of plant physiology, 1994, vol. 144, no. 3, pp. 307-313.

4. Kupper H., Seibert S., Parameswaran A. Fast, sensitive, and inexpensive alternative to analytical pigment HPLC: quantification of chlorophylls and carotenoids in crude extracts by fitting with Gauss peak spectra. Analytical chemistry. 2007, vol. 79, no. 20, pp. 7611-7627.

5. Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. Unificirovannaya ustanovka dlya laboratornyh issledovaniy mikrovodorosley. Voprosy sovremennoy al'gologii, 2017, № 1 (13). @@Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. Unified installation for laboratory research of microalgae. Questions of modern algology, 2017, no. 1 (13). (In Russ.)]

6. Zarrouk C. Contribution a l’etude d’une cyanophycee. Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthese de Spirulina maxima (Setch et Gardner). Geitler: Ph. D these, Paris, 1966, 114 p.

7. Gulyaev B.A., Litvin F.F. Pervaya i vtoraya proizvodnaya spektra pogloscheniya hlorofilla i soprovozhdayuschih pigmentov v kletkah vysshih rasteniy i vodorosley pri 20oS. Biofizika, 1970, t. 15, № 4, s. 670-680. @@Gulyaev B.A., Litvin F.F. The first and second derivatives of the absorption spectrum of chlorophyll and accompanying pigments in cells of higher plants and algae at 20oC. Biophysics, 1970, vol. 15, no. 4, pp. 670-680. (In Russ.)]

8. Bidigare R.R. et al. In vivo absorption properties of algal pigments. Ocean Optics X.International Society for Optics and Photonics, 1990, vol. 1302, pp. 290-302.

9. Hoepffner N., Sathyendranath S. Effect of pigment composition on absorption properties of phytoplankton. Mar. Ecol. Prog. Ser., 1991, vol. 73, no. 1, pp. 11-23.

10. Chernyshev D.N., Trenkenshu R.P. Model' rekonstrukcii spektra pogloscheniya krasnoy oblasti spirulina platensis in vivo po harakteristikam rastvorov hlorofilla a i fikobilinov. Aktual'nye voprosy biologicheskoy fiziki i himii, 2019, t. 4, №. 4, c. 467-471. @@Chernyshev D.N., Trenkenshu R.P. Model of reconstruction of the absorption spectrum of the red region of spirulina platensis in vivo according to the characteristics of solutions of chlorophyll a and phycobilins. Actual issues of biological physics and chemistry, 2019, vol. 4, no. 4, pp. 467-471. (In Russ.)]

11. Smith E.L. The action of sodium dodecyl sulfate on the chlorophyll-protein compound of the spinach leaf. The Journal of General Physiology, 1941, vol. 24, no. 5, p. 583.

12. Mantoura R.F.C., Wright S.W. Phytoplankton pigments in oceanography: guidelines to modern methods. Oceanographic Literature Review, 1997, vol. 10, no. 44, p. 1110.

13. Gevorgiz R.G., Nehoroshev M.V. Kolichestvennoe opredelenie massovoy doli summarnyh karotinoidov v suhoy biomasse Spirulina (Arthrospira) platensis North. Geitl. : uchebno-metodicheskoe posobie. RAN, In-t morskih biologicheskih issledovaniy im. A. O. Kovalevskogo. Sevastopol', 2017, 12 s. (Preprint RAN, IMBI). @@Gevorgiz R.G., Nekhoroshev M.V. Quantitative determination of the mass fraction of total carotenoids in dry biomass of Spirulina (Arthrospira) platensis North. Geitl. : educational and methodological manual. RAS, Institute of Marine Biological Research named after A. O. Kovalevsky. Sevastopol, 2017, 12 p. (Preprint RAS, IMBI). (In Russ.)]


Login or Create
* Forgot password?