Moscow, Moscow, Russian Federation
In this study we first took an integrated approach to the interaction of NO and H2S gases, produced by the bacterial cells within their signaling potencies in DNA repair responses to oxidative damages and control of bacterial biofilm production. In the study of crystalline nitrosyl iron complexes, it was found that combined treatment with an NO donor and hydrogen sulfide, under the condition of a 2.5-fold excess of H2S for each NO-donor group, stimulates an increase in NO-signaling activity due to the formation of more active DNICs with persulfide ligands in the cell, which, in contrast to DNIC with thiol ligands, give a characteristic “narrow” EPR signal with gaver = 2.03 (g⊥ = 2.032, g || = 2.02). Thereby, H2S acts as a mediator of NO signaling activity, thereby stimulating a significant increase in the level of soxS gene expression. The effect of the combined treatment on the level of sfiA gene expression was not found. This phenomenon causes a decrease in the level of oxidative stress during joint treatment with a NO donor and H2S, which has a significant effect on a decrease in the productivity of biofilm formation (on average by 30% relative to mono-treatment) and an increase in the dispersion indicators of mature biofilms (on average by 25% relative to mono-treatment). We suppose that our findings with the complex NO-donors with H2S application may be useful in solving the bacterial biofilm problems.
nitric oxide, hydrogen sulfide, biofilm, signaling functions
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