Histological studies in regenerative medicine are one of the most reliable methods for studying the variable reactivity and adaptive variability of cells and tissues under the influence of outer factors. However, processing with chemical fixatives, dehydration, making sections and staining of samples, being an integral part of histological sample preparation for subsequent optical microscopy, lead to the impossibility of obtaining the most accurate information about the vital state of the tissues of the sample under study. In this work, the method of acoustic microscopy is proposed as a safe, non-invasive and vital method for studying the microstructure of a spongeous scaffolds based on chitosan after implantation into a rat's body. Chitosan is a natural biocompatible polymer, has hemostatic activity, as well as antibacterial and antiseptic, due to which it is widely used in tissue engineering, therapy of infectious diseases, and disaster medicine [1]. The explants were examined by acoustic and optical microscopy using histological techniques. The rate of degradation of spongeous scaffolds based on chitosan under conditions of aseptic and purulent wounds was estimated. As a result of this study, the effectiveness of acoustic microscopy as a non-invasive, non-destructive tool for visualizing the results of the biodegradation of scaffolds based on chitosan has been demonstrated.
spongeous scaffold, chitosan, acoustic microscopy
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