The existing literature demonstrates the presence of antitumor effect of low-temperature plasma both in vitro on different cell lines and in vivo. This area has been intensively studied recently, however, research is complicated due to the large variety of research objects and insufficient knowledge of plasma jets. In this paper, the effect of a low-temperature plasma jet on the proliferation of HeLa tumor cells was studied. Results of experiments in different modes of cell culture processing and with variations in the parameters of the plasma source were presented. Low-temperature plasma jets were obtained using of a pulsed electric discharge sustained in the air flow at atmospheric pressure. In order to find optimal conditions, the repetition frequency of voltage pulses from 0.5 to 4.2 kHz, the exposure time from 30 to 240 s, the number of processing repetitions and the duration of the pause between repetitions (10 minutes, 24 hours, 72 hours) were varied. It was shown that this type of physical impact is able to suppress the growth of tumor cells up to 85% compared to the control group. This effect depends both on the repetition frequency of the discharge current pulses and on the time of exposure. The obtained data show the prospects of using low-temperature plasma jets to suppress the tumor cells proliferation.
low-temperature plasma, discharge in a gas stream, plasma jet, oncology, proliferation, HeLa
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