Institute of Botany ANAS
Baku, Azerbaijan
Baku, Azerbaijan
Baku, Azerbaijan
By using of microelectrode techniques, patterns of change in the potential (φm) and resistance (Rm) of the plasma membrane (PM) of Chara fragilis cells under the influence of Dandelion–Hypericum-Calendula (DHC) phytocomposition solutions were studied. The values of φm and Rm are widely scattered: -90 ÷ -300 mV and 1 ÷ 32.6 Ohm·m2, respectively under the standard conditions. The average value of φm was -183 ± 4.9 mV, Rm = 9.2 ± 1.2 Ohm·m2. Using the dependences of φm, Rm on the concentration of K+ in the external environment, the ranges of activation of the K+ - channels to the inward (KCİR) and outward rectification (KCOR) were determined. Under the action of the DHC phytocomposition, while the cell was in the medium with the concentration of the composite 10-1 or 1 mg/ml, we did not detect changes in φm and Rm for 25 min. Stimulation or suppression of transport processes under the influence of composites occurred only with the exclusion of DHC from the composition of the nutrient medium and with a stepwise increase in the concentration of the composition in the medium. The electrophysiological effects of the action of the DHC composition are discussed from the point of view of its action on the lipid phase of the plasma membrane of the experimental cells.
Chara fragilis, membrane potential, membrane resistance, phytoprotectors, H+ -pumps, K+ -channels
1. Gorkov V.A. et al. Herbal medicine for moderate depression with St. John's wort (analytical review). Psychiatry and psychopharmacotherapy, 2000, vol. 2, no. 6. (In Russ.)
2. Demidchik V.V. Membrane mechanisms of regulation of the activity of calcium ions in the cytoplasm of cells of higher plants. Proceedings of BSU, 2012, vol. 7, no. 1, pp. 99-105. (In Russ.)
3. Drobizhev M.Yu. et al. Gelarium Hypericum in the treatment of depression in the general somatic network. ConsiliumMedicum., Media Medica, 2002, vol. 4, no. 5. (In Russ.)
4. Musaev N.A. Biophysical mechanisms of modification of the transport properties of the plasma membrane of plant cells. Doctoral dissertation, Baku, 2012, 336 p. (In Russ.)
5. Perelman Ya.M. Analysis of dosage forms. Practical Guide. L. Medgiz, 1961, 616 p. (In Russ.].
6. Hasanova A.E., Musayev N.A. Bioelectric characteristics of Chara fragilis (Characeae) cells. Ukrainian Botanical Journal, M.G. Kholodny Institute of Botany, NAS of Ukraine, 2020, vol. 77, no. 5, pp. 398-404.
7. Ahmad V., Yasmeen S., Ali Z., Khan M., Choudhary M., Akhtar F., Miana G., Zahid M. Taraxacin, a new guaianolide from Taraxacumwallichii. J. Nat. Prod., 2000, vol. 63, pp. 1010-1011.
8. Shivasharan B., Nagakannan P., Thippeswamy B., Veerapur V. Protective Effect of Calendula officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats. Indian J. ClinBiochem., 2013, pp.292-298.
9. Choi J.H., Shin K.M., Kim N.Y., Lee Y.S., Kim H.J., Park H.J., Lee K.T. Taraxinic acid, a hydrolysate of sesquiterpene lactone glycoside from the Taraxacumcoreanum Nakai, induces the differentiation of human acute promyelocytic leukemia HL-60 cells. Biol. Pharm. Bull., 2002, vol. 25, pp. 1446-1450.
10. Hasanova A., Najafaliyeva Sh., Aliyeva P., Musayev N. Chara Fragilis cells - a new object for electrophysiology research. Journ. of Baku Engineering University, - Chemistry and Biology, 2017, vol. 1, no. 2, pp. 176-182.
11. Hogg J., Williams E.J., Jhonston R.I. A simplified method for measuring membrane resistances Nitellatranslucens. Biochim. et Biophys. Acta, 1968, vol. 150, p. 518.
12. Hogg J., Williams E.J., Jhonston R.I. Light intensity and the membrane parameters of Nitellatranslucens. Biochim. et Biophys. Acta, 1969, vol. 173, pp. 564-566.
13. Fonseca Y., Catini C., Vicentini F., Nomizo A. Protective effect of Calendula officinalis extract against UVB-induced oxidative stress in skin: Evaluation of reduced glutathione levels and matrix metalloproteinase secretion. Journal of Ethnopharmacology, 2009, no. 127, pp.596-601.
14. Yang D., Whang W., Kim I. The constituents of Taraxacum hallaisanensis roots. Arch. Pharmacol. Res., 1996, vol. 19, pp. 507-513.
15. Yun S., Cho H., Choi H. Anticoagulant from Taraxacum platycarpum. Biosci. Biotechnol. Biochem., 2002, vol. 66, pp. 1859-1864.
16. Prylutskyy Y.I., Vereshchaka I.V., Maznychenko A.V., Bulgakova N.V., Gonchar O.O., Kyzyma O.A., Ritter U., Scharff P., Tomiak T., Nozdrenko D.M., Mishchenko I.V., Kostyukov A.I. C60 fullerene as promising therapeutic agent for correcting and preventing skeletal muscle fatigue. Journal of Nanobiotechnology, 2017, vol. 15, no. 8, doi:https://doi.org/10.1186/s12951-016-0246-1.
