Yerevan, Armenia
Yerevan, Armenia
Yerevan, Armenia
Yerevan, Armenia
In this work the effect of millimeter range electromagnetic waves (MM EMW) on complex-formation peculiarities of Hoechst 33258 (H33258) with biomacromolecules – DNA and human serum albumin (HSA) has been studied by the methods of absorption and fluorescence spectroscopies. Spectroscopic characteristics of the complexes DNA-H33258 and HSA-H33258 were shown to depend on the irradiation frequency of MM EMW. It was also shown that at the irradiation by the frequency 41.8 GHz the effect of MM EMW is pronounced weaker, than at the irradiation by 50.3 GHz, which maintains the water role in the influence of MM EMW, since the frequency 50.3 GHz is resonant one for water. On the other hand, it was revealed that the irradiation of MM EMW leads to decreasing of H33258 affinity to biomacromolecules, since the MM EMW irradiation results in decreasing of the binding parameters of H33258 to DNA and HSA. Besides, the MM EMW irradiation results in the weakening of H33258 fluorescence by DNA and HSA. The important conclusion also is the fact that the irradiation of MM EMW by the frequency 41.8 GHz invokes a weak change of the aforementioned parameters of the complexes DNA-H33258, while it leaves a significant influence on the complexes HSA-H33258.
Hoechst 33258, DNA, human serum albumin, complex-formation, binding constant, fluorescence intensity quenching
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