Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Yakutsk, Yakutsk, Russian Federation
The nature of the delayed chlorophyll fluorescence (DF) in algae, which occurs already after the cessation of illumination due to the energy released during the reverse reactions of the primary photosynthesis photoproducts in the reaction center of the photosystem 2, is considered. The use of this fluorescence in algae for biotesting of contaminants is shown to be promising. The advantages of using DF for express assessment of water toxicity, especially drinking water, are short durations of tests and, accordingly, the minimized negative impact of physicochemical changes in the medium on the results, as well as the need of small amounts of test objects. The other advantage of DF is that only living cells with active photosynthetic reaction sites are measured, which increases the sensitivity of toxicity tests. This method provides additional information about the most important process in the cell - the energization of photosynthetic membranes and the formation of ATP associated with this.
photosynthesis, delayed fluorescence, biotesting, microalgae
1. Strehler B.L., Arnold W. Light production by green plants. J. Gen. Phys., 1951, vol. 34, pp. 809-820.
2. Govindjee Jursinic P.A. Photosynthesis and fast changes in light emission by green plants. In Photochemical and Photobiological Reviews. (Edited by K. C. Smith). Plenum Press., New York, 1979, vol. 4, pp. 125-205.
3. Goltsev V., Zaharieva I., Chernev P., Strasser R.J., Delayed fluorescence in photosynthesis. Photosynth. Res., 2009, vol. 101, pp. 217-232.
4. Matorin D.N., Venediktov P.S. Rubin A.B. Delayed fluorescence and its use to assess the state of the plant organism. Izd. AN SSSR, cer. biol., 1985, no. 4, pp. 508-520. (In Russ.)
5. Matorin D.N., Osipov V.A., Venediktov P.S., Rubin A.B. Delayed fluorescence of plants and algae. Theoretical and practical aspects. M.: Izd-vo Al`treks, 2011, 202 p. (In Russ.)
6. Berden-Zrimec M., Drinovec L., Zrimec A.M., Suggett D.J. et al. Delayed Fluorescence. Chlorophyll a fluorescence in aquatic sciences: methods and applications. Developments in applied/Phycology 4, Springer Science, 2011, pp. 293-309.
7. Krasnovsky A.A. Delayed fluorescence and phosphorescence of plant pigments. Photochem. Photobiol., 1982, vol. 36, pp. 773-774.
8. Matorin D.N., Yakovleva O.V. Plant photoluminescence. M. Izd-vo Al`treks, 2019, 256 p. (In Russ.)
9. Goltsev V.N., Ortoidze T.V., Sokolov J.N., Matorin D.N., Venediktov P.S. Delayed luminescence yield kinetics in flash illuminated green plants. Plant Sci. Lett., 1980, vol. 19, pp. 339-346.
10. Crofts A.R., Wright C.A., Fleischmann D.E. Energy conservation in the photochemical reactions of photosynthesis and its relation to delayed fluorescence. FEBS Lett., 1971, vol. 15, no. 1, pp. 89-100.
11. Ortoidze T.V., Borisevitch G.P., Venediktov P.S., Kononenko A.A., Matorin D.N., Rubin A.B. Electric field stimulation of the delayed fluorescence in dry films of chloroplasts and subchloroplast particles enriched in PS2. Biochim. Physiol. Pfanzen, 1979, vol. 174, no. 1, pp. 85-91.
12. Matorin D.N., Todorenko D.A., Seifullina N.Kh., Zayadan B.K., Rubin A.B. Effect of silver nanoparticles on the parameters of chlorophyll fluorescence and P700 reaction in the green alga Chlamydomonas reinhardtii. Microbiology, 2013, vol. 82, no. 6, pp. 862-867.
13. Matorin D.N., Plekhanov S.E., Bratkovskaia L.B., Iakovleva O.V., Alekseev A.A. The effect of phenols on the parameters of chlorophyll fluorescence and reaction of P700 in the green algae Scenedesmus quadricauda. Biofizika, 2014, vol. 59, no. 3, pp. 458-465.
14. Gabbasova D.T., Matorin D.N., Konyukhov I.V., Seifullina N.Kh, Zayadan B.K. Effect of chromate ions on marine microalgae Phaeodactylum tricornutum. Microbiology, 2017, vol. 86, no. 1, pp. 54-62.
15. Zayadan B.K., Sadvakasova A.K., Matorin D.N., Akmukhanova N.R., Kokocinski M., Timofeev N.P., Balouch Kh., Bauenova M.O. Effect of Cadmium Ions on Some Biophysical Parameters and Ultrastructure of Ankistrodesmus sp. B-11 Cells. Russian Journal of Plant Physiology, 2020, vol. 67, no. 5, pp. 845-854.
16. Vasil'ev I.R., Matorin D.N., Lyadsky V.V., Venediktov P.S. Multiple action sites for photosystem II herbicides as revealed by delayed fluorescence. Photosynth. Res., 1988, vol. 15, no. 1, pp. 33-39.
17. Dahse J., Matorin D.N., Liebermann B. A comparison of tentoxin action on the delayed fluorescence chloroplasts of Spinach, Chlorella and Anasystis. Biochem. Physiol. Pflanzen, 1986, vol. 181, pp. 137-146.
18. Grigoriev Yu.S., Stravinskene E.S. Method for measuring the relative indicator of delayed fluorescence of a chlorella alga (Chlorella vulgaris Beijer) culture for determining the toxicity of drinking, fresh natural and waste waters, water extracts from soils, soils, sewage sludge, production and consumption waste. PND F T 14.1:2:4.16-09 PND F T 16.1:2.3:3.14-09, 2014, 37 p. (In Russ.)
