Minsk, Belarus
Minsk, Belarus
Hydrogen peroxide, which is formed during cellular respiration and the action of physicochemical factors, is an important participant in intracellular regulatory processes. A moderate increase in the concentration of hydrogen peroxide activates the mechanisms associated with protection and adaptation, and at a high level of the oxidant, damage to cellular structures is observed. The work shows that under the action of hydrogen peroxide in micromolar concentrations, an increase in the structural stability of membranes and a decrease in the proportion of hemolyzed erythrocytes are observed upon destruction by hypochlorous acid, silver nanoparticles, and silver nitrate. A method for quantitative assessment of the regulation of the erythrocyte protective properties by hydrogen peroxide during hemolysis by various factors is proposed. It has been shown that the range of hydrogen peroxide concentrations at which an increase in the proportion of non-hemolyzed cells (the region of hormesis) is observed differs for various factors leading to destruction. Based on the developed mathematical model of cell destruction under the action of a damaging factor and hormesis dependences of the erythrocyte response to the action of hydrogen peroxide, the parameters characterizing the regulation of cellular adaptation processes were calculated. The proposed method for analyzing the protective properties of cells makes it possible to compare the adaptive properties of erythrocytes and determine the optimal conditions for their regulation by external factors.
erythrocytes, adaptation, resistance, hemolysis
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