Vladivostok, Vladivostok, Russian Federation
Vladivostok, Vladivostok, Russian Federation
Vladivostok, Vladivostok, Russian Federation
Quantum dots (QDs) are a new generation of fluorochromes with significant advantages over traditional organic dyes. QDs based on CdS are promising materials for optics, optoelectronics, biology, and medicine. QDs in the form of colloidal solutions are of great scientific and practical interest. CdS quantum dots were synthesized by chemical condensation in an aqueous solution using Yersinia pseudotuberculosis porin proteins, positively (chitosan) and negatively (carrageenan, lipopolysaccharide) charged polysaccharides. The maxima of the emission spectra for all samples were 450 nm, which indicates the same QD size; is determined by the size of the "cells" of the grid matrix, which limit the size of the QD. It was shown that the fluorescence intensity of QDs synthesized in LPS solutions was two times higher than that of other samples. The fluorescence of the samples and the maxima of the emission spectra (450 nm) are preserved during intensive dialysis against buffers, which indicates the stability of QDs and the possibility of using the obtained labeled preparations. Keeping samples at pH 3 leads to a significant decrease in fluorescence, especially for acidic polysaccharides. Anionic oxygen of phosphate, hydroxyl groups of sugar, nitrogen atoms can interact with metal ions, which are precursors (precursors) for CdS nanocrystals. It was found that the interaction with porin-specific IgG leads to a significant change in the luminescence intensity of QD-porin samples. This is of interest from the point of view of chemical sensing and opens the prospect of using QD-labeled porin-based nanostructures as biosensors.
porin, chitosan, carrageenan, lipopolysaccharide, cadmium sulfide, quantum dots, conjugation, luminescence
1. Novikova O.D., Naberezhnykh G.A., Sergeev A.A. Nanostructural biosensors based on bacterial membrane components. Biophysics, 2021, vol. 66, no. 4, pp. 668-683, doi:https://doi.org/10.31857/S0006302921040062. (In Russ.)
2. Murray C.B., Norris D.J., BawendiM.G. Synthesis and characterization of nearly monodisperse CdE (E = S, Se, Te) semiconductor nanocrystallites. J. Am. Chem. Soc., 1993, vol. 115, pp. 8706-8715, doi:https://doi.org/10.1021/ja00072a025.
3. Rosenthal S.J., McBridea J., Pennycook S.J., Feldman L.C. Synthesis, surface studies, composition and structural characterization of CdSe, core/shell and biologically active nanocrystals. Surf. Sci. Rep., 2007, vol. 62, pp. 111-157, doi:https://doi.org/10.1016/j.surfrep.2007.02.001.
4. Akerman M.E., Chan W., Laakkonen P., Bhatia S.N., Ruoslahti E. Nanocrystal targeting in vivo. PNAS, 2002, vol. 99, no. 20, pp. 12617-12621, doi:https://doi.org/10.1073/pnas.1524633993.
5. Abdellatif A.A.H., Younis M.A., Alsharidah M., Rugaie O., Tawfeek H.M. Biomedical Applications of Quantum Dots: Overview, Challenges, and Clinical Potential. Int. J. Nanomedicine, 2022, vol. 17, pp. 1951-1970, doi:https://doi.org/10.2147/IJN.S357980.
6. Yang G., Qin D., Zhang L. Controllable synthesis of protein-conjugated lead sulfide nanocubes by using bovine hemoglobin as a capping agent. J Nanopart.Res., 2014, vol. 16, no. 6, pp. 2438-2441, doi:https://doi.org/10.1007/s11051-014-2438-7.
7. Dickerson M.B., Sandhage K.H., Naik R.R. Protein- and Peptide-Directed Syntheses of Inorganic Materials. Chem. Rev., 2008, vol. 108, no. 11, pp 4935-497, doi:https://doi.org/10.1021/cr8002328.
8. Naberezhnykh G.A., Sergeev A.A., Portnyagina O.Yu., Chistyulin D.K., Sidorin E.V., Novikovaa O.D. Bioconjugation of colloidal quantum dots of cadmium sulfides and supramolecular structures of porin protein from bacteria of the genus Yersinia. Obtaining and characterization. Russian Journal of Biological Physics and Chemistry, 2020, vol. 5, no. 4, pp. 652-658. (In Russ.)
9. Novikova O.D., Vakorina T.I., Khomenko V.A., Likhatskaya G.N., Kim N.Yu, Emelyanenko V.I, Kuznetsova S.M., Solov’eva T.F. Influence of cultivation conditions on spatial structure and functional activity of OmpF like porin from outer membrane of Yersinia pseudotuberculosis. Biochemistry, 2008, vol. 73, no. 2, pp. 173-184, doi:https://doi.org/10.1134/s0006297908020041. (In Russ.)
10. Naberezhnykh G.A., Gorbach V.I., Likhatskaya G.N. Interaction of Chitosans and N-acylated Chitosan Derivatives with Lipopolysaccharides of Gram-Negative Bacteria. Biokhimiya, 2008, vol. 73, no. 4, pp. 530-541, doi:https://doi.org/10.1134/s0006297908040081.
11. Galanos O. Luderitz, Westphal O. A new method for the extraction of R lipopolysaccharides. Eur. J. Biochem., 1969, vol. 9, c. 245-249, doi:https://doi.org/10.1111/j.1432-1033.1969.tb00601.x.
12. Yermak I.M., Kim Y.H., Titlyanov E.A., Isakov V.V., Solov’eva T.F. Chemical structure and gel properties of carrageenan from algae belonging to the Gigartinaceae and Tichocapaceae, collected from the Russian Pacific coast. J. Applied Phycology, 1999, vol. 11, pp. 41-48, doi:https://doi.org/10.1023/A:1008071925884.
13. Naberezhnykh G.A., Khomenko V.A., Solov’eva T.F., Novikova O.D., Karpenko A.A. The formation of ordered structures of bacterial porins in a lipid bilayer and the analysis of their morphology by atomic force microscopy. Biophysics, 2019, vol. 64, no. 6, pp. 901-907, doi:https://doi.org/10.1134/S0006302919060097.
14. Razumov V.F. Fundamental and applied aspects of luminescence of colloidal quantum dots. Advances Physical Science, 2016, vol. 186, no. 2, pp. 1368-1376, doi:https://doi.org/10.3367/UFNr.2016.03.037861. (In Russ.)
