COMPARATIVE ANALYSIS OF THE INHIBITORY EFFECTS OF THE HYPOMAGNETIC FIELD AND COMBINED MAGNETIC FIELDS ON THE PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS) BY NEUTROFILS
Abstract and keywords
Abstract (English):
The article reveals that a decrease in the background production of reactive oxygen species (ROS) in the peritoneal neutrophils of mice after a short-term (40 minutes) stay in hypomagnetic conditions (residual field  10 nT) at physiological temperatures, detected by the method of lucigenin-dependent chemiluminescence, is not accompanied by a violation of chemiluminescent response to respiratory burst activators: formylated peptide N-formyl-Met-Leu-Phe (fMLF) and phorbol ester of phorbol-12-meristat-13-acetate (PMA). These results were obtained by activated chemiluminescence using lucigenin or luminol and various combinations of ROS production activators (PMA and/or fMLF). In contrast, the action of combined parallel constant (induction 60 μT) and alternating (amplitude range 60-180 nT, frequency 49.5 Hz) magnetic fields (CMF) leads to a decrease in the chemiluminescent response to these activators. These data indicate different sources of ROS that respond to certain modes of CMF and hypomagnetic field in neutrophils. The conducted research and the previously obtained results enable to exclude the systems that control the respiratory burst in neutrophils from the main targets and acceptors that respond to short-term deprivation of the magnetic field.

Keywords:
hypomagnetic field, neutrophils, reactive oxygen species, lucigenin, luminol, chemiluminescence
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References

1. Novikov V.V., Yablokova E.V., Fesenko E.E. The effect of a «zero» magnetic field on the production of reactive oxygen species in neutrophils. Biophysics (Moscow), 2018, vol. 63, no. 3, pp. 365-368 (In Russ.).

2. Novikov V.V., Yablokova E.V., Shaev I.A., Fesenko E.E. Decreased production of the superoxide anion radical in neutrophils exposed to a near-null magnetic field. Biophysics (Moscow), 2020, vol. 65, no. 4, pp. 625-630 (In Russ.).

3. Aasen T.B., Bolann B., Glette J., Ulvik R.J., Schreiner A. Lucigenin-dependent chemiluminescence in mononuclear phagocytes. Role of superoxide anion. Scand J Clin Lab Invest, 1987, vol. 47, pp. 673-679.

4. Novikov V.V., Yablokova E.V., Valeeva E.R., Fesenko E.E. On the molecular mechanisms of the effect of a zero magnetic field on the production of reactive oxygen species in inactivated neutrophils. Biophysics (Moscow), 2019, vol. 64, no. 4, pp. 371-375 (In Russ.).

5. Shaev I.A., Novikov V.V. Influence of weak alternating magnetic fields on neutrophilic granulocytes. Analytical review. Physics of Biology and Medicine, 2023, no. 1, pp. 26-43 (In Russ.).

6. Cuppen J., Gradinaru C., Raap-van Sleuwen B., de Wit A., van der Vegt T., Savelkoul H. LF-EMF Compound block type signal activates human neutrophilic granulocytes in vivo. Bioelectromagnetics, 2022, vol. 43, no. 5, pp. 309-316.

7. Poniedzialek B., Rzymski P., Nawrocka-Bogusz H., Jaroszyk F., Wiktorowicz K. The effect of electromagnetic field on reactive oxygen species production in human neutrophils in vitro. Electromagn Biol Med, 2013, vol. 32, no. 3, pp. 333-341.

8. Belova N.A., Potselueva M.M., Srebnitskaya L.K., Znobishcheva A.V., Lednev V.V. Effects of weak magnetic fields on the production of reactive oxygen species in peritoneal neutrophils in mice. Biofizika, 2010, vol. 55, no. 4, pp. 657-663 (In Russ.).

9. Novikov V.V., Yablokova E.V., Fesenko E.E. A decrease of the respiratory burst in neutrophils after exposure to weak combined magnetic fields of a certain duration. Biophysics (Moscow), 2020, vol. 65, no. 1, pp. 82-87 (In Russ.).

10. Proskurnina E.V., Sozarukova M.M., Polimova A.M., Prudnikova M.A., Ametov A.S., Vladimirov Yu.A. A new chemiluminescent method for assessing the functional activity of neutrophils in the examination of patients with type 2 diabetes mellitus. Bulletin of Experimental Biology and Medicine, 2016, vol. 161, no. 2, pp. 288-291 (In Russ.).

11. Rossi F., Bellavite P., Berton G. et al. The respiratory burst of phagocytic cells: facts and problems. Adv. Exp. Med. Biol., 1982, vol. 141, pp. 283-322.

12. Safronova V.G., Gabdulkhakova A.G., Miller A.V. et al. Variability of insulin action on respiratory burst in neutrophils. The role of tyrosine kinases and phosphatases. Biokhimiya, 2001, vol. 66, pp. 840-849 (In Russ.).


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