Saint Petersburg, St. Petersburg, Russian Federation
Saint Petersburg, St. Petersburg, Russian Federation
Saint Petersburg, St. Petersburg, Russian Federation
The usage of the DNA molecule in the design of new systems for their application in the therapy of various diseases has generated a lot of interest recently. Nucleic acids are used not only as factors of the influence on sick organism at the genetic level (for example, as gene vectors), but also as tools for the transfer of various biologically active agents into target cells. The high-molecular DNA can be applied in this case. An important step in the creation of such structures is DNA packaging. It ensures the penetration of DNA structures through membranes and also protects DNA from the action of nucleases. Various cationic polymers are used as compacting agents. Inclusion of noble metal nanoparticles into compact DNA-polymer structures can expand the scope of such constructions in medicine due to the catalytic and optical properties of metal nanoparticles. The aim of this work was to study the properties of the systems formed by DNA compaction using a synthetic polymer conjugated with silver nanoparticles. The result of adding a luminescent dye to such structures is considered. In this work, the well-known dye ethidium bromide is used for this purpose. The physicochemical properties of the formed structures were studied by viscometry, UV spectrophotometry, and luminescence spectroscopy.
DNA, silver nanoparticles, DNA-polymer particles with inclusion of a dye and metal nanoparticles
1. Lv Z., Zhu Yi., Li F. DNA Functional Nanomaterials for Controlled Delivery of Nucleic Acid-Based Drugs. Front. Bioeng. Biotechnol, 2021, vol. 9, p. 720291, doi:https://doi.org/10.3389/fbioe,2021.720291.
2. Bege M., Borbás A. The Medicinal Chemistry of Artificial Nucleic Acids and Therapeutic Oligonucleotides. Pharmaceuticals, 2022, vol. 15, p. 909, doi:https://doi.org/10.3390/ph15080909.
3. Wu X., Wu T., Lu J., Ding B. Gene therapy based on nucleic acid nanostructure. Adv. Healthc. Mater, 2020, vol. 9, e2001046, doi:https://doi.org/10.1002/adhm.202001046.
4. Frisman E.V., Schagina L.V., Vorobiev V.I. A glass rotation viscometer. Biorheology, 1965, vol. 2, pp. 189-194.
5. Eigner J., Doty P. The native, denatured and renatured states of deoxyribonucleic acid. Journal of Molecular Biology, 1965, vol. 12, pp. 549-580, doi:https://doi.org/10.1016/S0022-2836(65)80312-6.
6. Shvedchenko D.O., Nekrasova T.N., Nazarova O.V., Buffat P.A., Suvorova E.I. Mechanism of formation of silver nanoparticles in MAG–DMAEMA copolymer aqueous solutions. J Nanopart Res, 2015, vol. 17, pp. 1-13, doi:https://doi.org/10.1007/s11051-015-3083-5.
