Minsk, Belarus
Minsk, Belarus
In the present work, were analyzed the redox activity of cerium dioxide (CDC) nanoparticles of various sizes and studied their ability to regulate the adaptive mechanisms of erythrocytes in the presence of hydrogen peroxide. Cerium dioxide nanoparticles of various sizes were synthesized by homogeneous precipitation in the presence of hexamethylenetetramine at a temperature of 60°C and various synthesis times. The redox properties of cerium dioxide nanoparticles were evaluated spectrofluorimetrically based on the change in the rate of oxidation of 2',7'-dichlorodihydrofluorescein with hydrogen peroxide. It was shown that of the three sizes studied, the largest nanoparticles have the highest stability and aggregation resistance. Using the method of scanning electron microscopy, it was found that these nanoparticles have a predominantly spherical shape with an average diameter of 50 nm and do not contain impurity atoms. As a result of studies in cell-free solutions, it was found that NDC exhibit antioxidant properties and reduce the rate of oxidation of 2',7'-dichlorodihydrofluorescein by hydrogen peroxide. Preliminary incubation of erythrocytes with hydrogen peroxide at a concentration of 100–300 μM makes it possible to reduce the proportion of hemolyzed cells upon destruction by silver nitrate. The use of cerium dioxide nanoparticles leads to a potentiation of the protective effect and a shift in the hormesis region towards lower concentrations of hydrogen peroxide.
Cerium dioxide nanoparticles, erythrocytes, hydrogen peroxide, adaptation
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