OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS

S Y. Huseynova; T M Huseynov; M Z Dadashov

UDC

577

OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE IN VITRO TESTS OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS

АА Аа

Published in Russian Journal of Biological Physics and Chemisrty · Pages 189–195 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

Received: 20.03.2018 Accepted: 20.03.2018 Published: 25.03.2018 Language of publication: RUS

Authors

S Y. Huseynova

T M Huseynov

M Z Dadashov

S Y. Huseynova

Institute of Biophysics, NAS of Azerbaijan

T M Huseynov

Institute of Biophysics, NAS of Azerbaijan

M Z Dadashov

Institute of Biophysics, NAS of Azerbaijan

ABSTRACT

The work explored the influence of moderately chronic doses of NaNO2 on the oxidative modification of Hb and RBC, changes the activity of catalase, glutathione peroxidase (GP) and the intensity of peroxide oxidation of lipids (LPO). Analysis of the spectral lines of Raman Spectroscopy shows that the ability of the Hb to bind O2 I1580/I1548 in a suspension of erythrocytes treated with NaNO2 is significantly higher than control and this effect directly depends on the concentration of NaNO2 and significant even at the minimum concentrations of NaNO2 (0,07 mm), and relative ability of Hb to give ligands I1375/I1580 is also dose dependent but in the opposite direction. Manifestations of symmetrical and asymmetrical vibrations of the pyrrole rings of Hb (I1375/I1172) increase with an increasing nitrite dose by ~ 14 %, which indicates the presence of conformational changes in the pyrrole rings associated with the oxidative modification of Hb. MetHb accumulation also dose-depend and reaches its maximum at 30-40 minutes, this is accompanied by the intensification of processes of LPO and the increasing share of membrane bound Hb. It is characteristic that the development of oxidative process is accompanied by a decrease in catalase activity and the activity of GP changes ambiguously and multiple grows with increased NaNO2 contents. Noting these facts, we can say that the already relatively small concentrations of nitrite of sodium can affect the conformation of Hb, resulting in a change in his affinity for O2. This stimulates oxidative modification of Hb, which is reflected in the increasing accumulation of metHb and loss of activities of catalase and GP, and at high concentrations of nitrite to increase the activity of GP.

KEYWORDS

erythrocytes, hemoglobin, catalase, glutathionperoxidase, LPO, sodium nitrite

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Huseynova S. Y., Huseynov T. M., Dadashov M. Z. OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS // . 2018. pp. 189-195. (Date of access 20.08.2026).

MLA

S Y. Huseynova, Huseynov T. M., Dadashov M. Z.. "OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS." (2018): 189-195.

APA

Huseynova S. Y., Huseynov T. M., Dadashov M. Z. (2018). OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS. , 189-195.

Chicago

S Y. Huseynova, Huseynov T. M., Dadashov M. Z.. "OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS." , no. (2018): 189-195.

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{{cite journal | author = Huseynova S. Y., Huseynov T. M., Dadashov M. Z. | title = OXIDATIVE MODIFICATION OF ERYTHROCYTES MODERATELY DOSES OF SODIUM NITRITE in vitro TESTS | journal = | year = 2018 | pages = 189-195 }}

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