PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY

D V Grigorieva; M S Terekhova; E V Shamova; N A Efremov; E V Malyushkova; I V Semak; S N Cherenkevich; A V Sokolov; O M Panasenko; I V Gorudko

UDC

577

PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY

Published in Russian Journal of Biological Physics and Chemisrty · Pages 598–605 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

Received: 20.09.2018 Accepted: 20.09.2018 Published: 25.09.2018 Language of publication: RUS

Authors

D V Grigorieva

M S Terekhova

E V Shamova

N A Efremov

E V Malyushkova

I V Semak

S N Cherenkevich

A V Sokolov

O M Panasenko

I V Gorudko

D V Grigorieva

Belarusian State University

M S Terekhova

Belarusian State University

E V Shamova

Belarusian State University

N A Efremov

«N.N. Alexandrov Republican Scientific and Practical Center of Oncology and Medical Radiology

E V Malyushkova

Belarusian State University

I V Semak

Belarusian State University

S N Cherenkevich

Belarusian State University

A V Sokolov

Research Institute of Experimental Medicine

O M Panasenko

Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency

I V Gorudko

Belarusian State University

ABSTRACT

It was established that recombinant human lactoferrin (rhLf), isolated from transgenic goats milk, binds to human neutrophils. This binding leads to an increase in intracellular Ca2+ due to the mobilization of Ca2+ from the endoplasmic reticulum and influx of extracellular Ca2+ through the store-operating calcium channels and T-type calcium channels. It was found that expose of neutrophils to rhLf lead to actin cytoskeleton reorganization and increase in intracellular protein tyrosine phosphorylation. RhLf augmented fMLP-induced respiratory burst of neutrophils. Together, these finding indicate that rhLf has a modulating effect on the functional neutrophil properties. Obtained results can be used to regulate the body's immune response, aimed to enhance the functional activity of neutrophils in pathologies associated with decreased killer capacity of these cells.

KEYWORDS

recombinant human lactoferrin, neutrophils, respiratory burst, calcium ions, tyrosine kinases, actin cytoskeleton

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GOST

Grigorieva D. V., Terekhova M. S., Shamova E. V., Efremov N. A., Malyushkova E. V., Semak I. V., Cherenkevich S. N., Sokolov A. V., Panasenko O. M., Gorudko I. V. PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY // . 2018. pp. 598-605. (Date of access 20.08.2026).

MLA

D V Grigorieva, Terekhova M. S., Shamova E. V., Efremov N. A., Malyushkova E. V., Semak I. V., Cherenkevich S. N., Sokolov A. V., Panasenko O. M., Gorudko I. V.. "PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY." (2018): 598-605.

APA

Grigorieva D. V., Terekhova M. S., Shamova E. V., Efremov N. A., Malyushkova E. V., Semak I. V., Cherenkevich S. N., Sokolov A. V., Panasenko O. M., Gorudko I. V. (2018). PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY. , 598-605.

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WIKI

{{cite journal | author = Grigorieva D. V., Terekhova M. S., Shamova E. V., Efremov N. A., Malyushkova E. V., Semak I. V., Cherenkevich S. N., Sokolov A. V., Panasenko O. M., Gorudko I. V. | title = PRIMING EFFECT OF RECOMBINANT HUMAN LACTOFERRIN ON THE NEUTROPHIL ACTIVITY | journal = | year = 2018 | pages = 598-605 }}

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