Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic

G. Naberezhnykh; A. Sergeev; O. Portnyagina; D. Chistyulin; E V Sidorin; O. Novikova

UDC

577

BIO-CONJUGATION OF COLLOIDAL QUANTUM DOTS OF CADMIUM SULPHIDES AND SUPROMOLECULAR STRUCTURES OF PROTEIN-PORIN FROM BACTERIA OF THE GENUS YERSINIA. OBTAINING AND CHARACTERISTIC Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic

АА Аа

Published in Russian Journal of Biological Physics and Chemisrty · Pages 652–658 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

Received: 20.12.2020 Accepted: 20.12.2020 Published: 25.12.2020 Language of publication: RUS

Authors

G. Naberezhnykh

A. Sergeev

O. Portnyagina

D. Chistyulin

E V Sidorin

O. Novikova

G. Naberezhnykh

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Russian Federation · Vladivostok

A. Sergeev

Institute of Automation and Control Processes Far Eastern Branch of the Russian Academy of Sciences Russian Federation · Vladivostok

O. Portnyagina

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Russian Federation · Vladivostok

D. Chistyulin

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Russian Federation · Vladivostok

E V Sidorin

Elyakov Pacific Institute of Bioorganic Chemistry, FarEastern Branch of the Russian Academy of Sciences

O. Novikova

G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Russian Federation · Vladivostok

ABSTRACT

Cadmium sulfide fluorescence quantum dots (QDs) are promising materials for optics, optoelectronics, biology, and medicine. In recent years, significant progress has been achieved in the use of nanomaterials to create biosensors, including based on protein structures ones. Among others, native or artificially constructed biosensors based on ion-conducting channels are a very promising class of nanostructures. In this work, various approaches were tested for the formation of ordered supramolecular structures of Yersinia porins (Yersinia pseudotuberculosis and Y. ruckeri) labeled with QDs: (1) conjugation of the porin matrix with stabilized QDs previously obtained and (2) QDs synthesis in a porin matrix with supporting lipid bilayer preformed on mica. The sizes of QDs and their conjugates with proteins were measured using the method of dynamic light scattering (directly in an aqueous suspension). The surface morphology of the samples was studied by atomic force microscopy. To characterize the optical properties of the obtained conjugates scanning fluorescence spectroscopy was used. It was found that the luminescence intensity of bioconjugates substantially depends on the method of QDs preparation and on protein sample used in matrix (isolated porin or the porin-peptidoglycan complex). It was shown that, depending on the matrix type, a shift in the fundamental absorption edge of QDs is observed. This indicates the formation of QDs of different diameters and, therefore, the possibility of controlling their sizes by varying the structure of the protein matrix. The data obtained open the prospect of using QDs labeled nanostructures based on bacterial porins as biosensors.

KEYWORDS

porin, cadmium sulfide, quantum dots, conjugation, luminescence

Funding

Rabota vypolnena pri finansovoy podderzhke Rossiyskogo fonda fundamental'nyh issledovaniy (proekt № 19-03-00318).

1. Razumov V.F. Fundamental'nye i prikladnye aspekty lyuminescencii kolloidnyh kvantovyh tochek. Uspehi fizicheskih nauk, 2016, t. 186, № 12, s. 1368-1376. @@Razumov V.F. Fundamental and applied aspects of luminescence of colloidal quantum dots. Advances Physical Science, 2016, vol. 186, no. 2, pp. 1368-1376. (In Russ.)

2. Wang Y., Herron N.J. Nanometer-sized semiconductor clusters: materials synthesis, quantum size effects, and photophysical properties. Phys. Chem., 1991, vol. 95, no. 2, pp. 525 -532.

3. Rosenbusch J.P. Characterization of the major envelope protein from Escherichia coli. Regular arrangement on the peptidoglycan and unusual dodecylsulfate binding. J. Biol. Chem., 1974, vol. 249, pp. 8019-8029.

4. Novikova O.D., Vakorina T.N., Homenko V.A., Lihackaya G.N., Kim N.Yu., Emel'yanenko V.I., Kuznecova S.M., Solov'eva T.F. Vliyanie usloviy kul'tivirovaniya na prostranstvennuyu strukturu i funkcional'nuyu aktivnost' OmpF-podobnogo porina iz naruzhnoy membrany Yersinia pseudotuberculosis. Biohimiya, 2008, t. 73, № 2, s. 173-184. @@Novikova O.D., Vakorina T.I., Khomenko V.A., Likhatskaya G.N., Kim N.Yu,. Emelyanenko V.I, Kuznetsova S.M., Solov’eva T.F. Influence of cultivation conditions on spatial structure and functional activity of OmpF like porin from outer membrane of Yersinia pseudotuberculosis. Biochemistry, 2008, vol. 73, no. 2, pp. 173-184. (In Russ.)

5. Garavito R.M., Rosenbusch J.P. Isolation and crystallization of bacterial porin. Methods Enzymol, 1986, vol. 125, pp. 309-328.

6. Qi Xiao, Chong Xiao. Surface-defect-states photoluminescence in CdS nanocrystals prepared by one-step aqueous synthesis method Applied Surface. Science, 2009, vol. 255, pp. 7111-7114.

7. Naberezhnyh G.A., Karpenko A.A., Homenko. V.A., Solov'eva T.F., Novikova O.D. Poluchenie uporyadochennyh struktur bakterial'nyh porinov v lipidnom bisloe i issledovanie ih morfologii metodom atomno-silovoy mikroskopii. Biofizika, 2019, t. 64, № 6, s. 1107-1114. @@Naberezhnykh G.A, Karpenko A.A., Khomenko V.A., Solov’eva T.F., Novikova O.D. The formation of Ordered Structures of Bacterial Porins in a Lipid Bilayer and Analysis of Their Morphology by Atomic Force Microscopy. Biophysics, 2019, vol. 64, no. 6, pp. 901-907.

8. Rempel A., Magerl A. Non-periodicity in nanoparticles with close-packed structures. Acta Crystal. Section A, 2010, vol. 6, no. 4, pp. 479-483.

9. Voznyy O. Mobile Surface Traps in CdSe Nanocrystals with Carboxylic Acid Ligands. J. Phys. Chem. C, 2011, vol. 115, no. 32, pp. 15927-15932.

This work is licensed under Creative Commons Attribution 4.0 International

JATS XML

Executive Secretary

Dmitriy Voronin

dpvoronin@sevsu.ru

Chief Editor

Maksim Evstigneev

Science Editor

Vsevolod A. Tverdislov

tverdislov@mail.ru

This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Naberezhnykh G. A., Sergeev A. A., Portnyagina O. Y., Chistyulin D. K., Sidorin E. V., Novikova O. D. Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic // . 2020. pp. 652-658. (Date of access 19.08.2026).

MLA

G. Naberezhnykh, Sergeev A. A., Portnyagina O. Y., Chistyulin D. K., Sidorin E. V., Novikova O. D.. "Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic." (2020): 652-658.

APA

Naberezhnykh G. A., Sergeev A. A., Portnyagina O. Y., Chistyulin D. K., Sidorin E. V., Novikova O. D. (2020). Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic. , 652-658.

Chicago

WIKI

{{cite journal | author = Naberezhnykh G. A., Sergeev A. A., Portnyagina O. Y., Chistyulin D. K., Sidorin E. V., Novikova O. D. | title = Bio-conjugation of colloidal quantum dots of cadmium sulphides and supromolecular structures of protein-porin from bacteria of the genus Yersinia. Obtaining and characteristic | journal = | year = 2020 | pages = 652-658 }}

Copy

Export: BibTeX EndNote RefMan RusMARC

Confirmation