Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species

D I Grachev; K B Shumaev; V. Medvedeva; A S Fefler; O V Kosmachevskaya; E K Ruuge

UDC

577

INTERACTION OF VARIOUS NITROSYL COMPLEXES OF HEMOGLOBIN WITH REACTIVE OXYGEN AND NITROGEN SPECIES Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species

АА Аа

Published in Russian Journal of Biological Physics and Chemisrty · Pages 477–481 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

Received: 20.09.2021 Accepted: 20.09.2021 Published: 25.09.2021 Language of publication: RUS

Authors

D I Grachev

K B Shumaev

V. Medvedeva

A S Fefler

O V Kosmachevskaya

E K Ruuge

D I Grachev

Lomonosov Moscow State University; National Medical Research Center of Cardiology of the Ministry of Health of Russian Federation

K B Shumaev

National Medical Research Center of Cardiology of the Ministry of Health of Russian Federation; Federal Research Center “Fundamentals of Biotechnology” Russian Academy of Sciences

V. Medvedeva

Lomonosov Moscow State University Faculty of Physics · Russian Federation · Moscow

E.I. Chazov National Medical Research Centre of Cardiology Russian Federation · Moscow

A S Fefler

Lomonosov Moscow State University

O V Kosmachevskaya

Federal Research Center “Fundamentals of Biotechnology” Russian Academy of Sciences

E K Ruuge

Lomonosov Moscow State University; National Medical Research Center of Cardiology of the Ministry of Health of Russian Federation

ABSTRACT

In this work, we studied the effect of reactive oxygen and nitrogen forms on the complexes of NO with hemoglobin under conditions simulating various physiological and pathophysiological conditions. It is well known that reactive oxygen species, reactive halogen species (hypochlorite), reactive nitrogen species (peroxynitrite and nitryl chloride) and other free radicals are involved in inflammatory reactions and in the pathogenesis of various diseases of the most important systems of the human and animal body. Stabilized forms of nitric oxide (NO) bound to hemoglobin: complexes of NO with heme iron (HbNO) and dinitrosyl iron complexes (DNIC) formed on thiol groups of cysteine residues of hemoglobin (Hb-DNIC) are important physiological forms of nitric oxide. Of particular interest for research is the participation of such NO complexes in processes associated with oxidative stress. We showed that peroxynitrite and hypochlorite quantitatively destroy Hb-DNIC, and in the case of hypochlorite, nitrosylation of the hemoglobin heme group does not occur. In addition, the antioxidant effect of Hb-DNIC was shown when interacting with alkoxyl and alkyl peroxide radicals obtained by adding tert-butyl hydroperoxide to a methemoglobin solution, which simulates peroxidation that occurs during oxidative stress. The data obtained make it possible to improve understanding of the role of complexes of nitric oxide with hemoglobin in the balance between antioxidant and prooxidant systems in the body.

KEYWORDS

reactive oxygen and nitrogen species, hemoglobin, EPR spectroscopy

Funding

Rabota byla vypolnena pri finansovoy podderzhke Rossiyskogo fonda fundamental'nyh issledovaniy, grant 19-015-00444.

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Dmitriy Voronin

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Maksim Evstigneev

Science Editor

Vsevolod A. Tverdislov

tverdislov@mail.ru

This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Grachev D. I., Shumaev K. B., Medvedeva V. A., Fefler A. S., Kosmachevskaya O. V., Ruuge E. K. Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species // . 2021. pp. 477-481. (Date of access 20.08.2026).

MLA

D I Grachev, Shumaev K. B., Medvedeva V. A., Fefler A. S., Kosmachevskaya O. V., Ruuge E. K.. "Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species." (2021): 477-481.

APA

Grachev D. I., Shumaev K. B., Medvedeva V. A., Fefler A. S., Kosmachevskaya O. V., Ruuge E. K. (2021). Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species. , 477-481.

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{{cite journal | author = Grachev D. I., Shumaev K. B., Medvedeva V. A., Fefler A. S., Kosmachevskaya O. V., Ruuge E. K. | title = Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species | journal = | year = 2021 | pages = 477-481 }}

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INTERACTION OF VARIOUS NITROSYL COMPLEXES OF HEMOGLOBIN WITH REACTIVE OXYGEN AND NITROGEN SPECIES Interaction of various nitrosyl complexes of hemoglobin with reactive oxygen and nitrogen species

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