THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO

E. Malinovskaya; E. Savinova; V. Sergeeva; E. Ershova; E. Proskurnina; L. Kameneva; O. Dolgikh; N. Veiko; O. Kraevaya; A. Zhilenkov; P. Troshin; S. Kostyuk

doi:10.29039/rusjbpc.2022.0523

UDC

577

THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFS) IN VITRO THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO

АА Аа

Published in Russian Journal of Biological Physics and Chemisrty · Pages 325–333 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

DOI: https://doi.org/10.29039/rusjbpc.2022.0523

Received: 20.06.2022 Accepted: 20.06.2022 Published: 25.06.2022

Authors

E. Malinovskaya

E. Savinova

V. Sergeeva

E. Ershova

E. Proskurnina

L. Kameneva

O. Dolgikh

N. Veiko

O. Kraevaya

A. Zhilenkov

P. Troshin

S. Kostyuk

E. Malinovskaya

Research Centre for Medical Genetics Russian Federation · Moscow

E. Savinova

Research Centre for Medical Genetics Russian Federation · Moscow

V. Sergeeva

Research Centre for Medical Genetics Russian Federation · Moscow

E. Ershova

Research Centre for Medical Genetics Russian Federation · Moscow

Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology Russian Federation · Moscow

E. Proskurnina

Research Centre for Medical Genetics Russian Federation · Moscow

L. Kameneva

Research Centre for Medical Genetics Russian Federation · Moscow

O. Dolgikh

Research Centre for Medical Genetics Russian Federation · Moscow

N. Veiko

Research Centre for Medical Genetics Russian Federation · Moscow

O. Kraevaya

FRC PCPMC RAS Russian Federation · Chernogolovka

A. Zhilenkov

FRC PCPMC RAS Russian Federation · Chernogolovka

P. Troshin

FRC PCPMC RAS Russian Federation · Chernogolovka

S. Kostyuk

Research Centre for Medical Genetics Russian Federation · Moscow

Federal Scientific and Clinical Center of Medical Rehabilitation and Balneology of the Federal Medical and Biological Agency of Russia Russian Federation · Moscow

ABSTRACT

The paper considers the effect of two water-soluble C60 fullerene derivatives modified with amino acid residues on the level of reactive oxygen species (ROS) in cultured human cells and on the transcriptional activity of genes that regulate pro- and antioxidant activity of cells. C60 fullerene derivatives with attached serine and phenibut amino acid residues were synthesized from C60Cl6 chlorofullerenes. Human embryonic lung fibroblast cultures were used in in vitro experiments. Various concentrations of fullerene derivatives were added to the culture medium, the cells were cultured in the presence of compounds from 1 to 72 hours. The level of ROS was determined using the dye 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA), which is oxidized by free radicals to form fluorescent 2,7-dichlorofluorescein (DCF), by fluorescence microscopy, flow cytometry, and using a plate reader. The level of protein expression was determined by flow cytometry using specific antibodies. The level of gene expression was assessed by real-time polymerase chain reaction. It was shown that when the studied compounds are introduced into the cellular environment, they intensively absorb reactive oxygen species due to double conjugated bonds in the framework. At the same time, fullerene derivatives contribute to the development of secondary oxidative stress in cells 24 hours after administration. This effect occurs due to the activation of the NOX4 enzyme. In the cells of human embryonic lung fibroblasts, which were injected with the studied C60 fullerene derivatives, a correlation was found between the average value of the NOX4 enzyme protein and the level of reactive oxygen species. The lack of activation of the antioxidant transcription factor NRF2 upon incubation of human embryonic lung fibroblasts in the presence of C60 fullerene derivatives contributes to the development of secondary oxidative stress in cells.

KEYWORDS

water-soluble derivatives of fullerene C60, reactive oxygen species, human embryonic lung fibroblasts, oxidative stress

Funding

Rabota vypolnena pri podderzhke grantov RNF: proekt 19-13-00411-P (sintez F1 i F17) i proekt 18-15-00437 P (vliyanie F1 i F17 na FLECh).

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This work is licensed under Creative Commons Attribution 4.0 International

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Dmitriy Voronin

dpvoronin@sevsu.ru

Chief Editor

Maksim Evstigneev

Science Editor

Vsevolod A. Tverdislov

tverdislov@mail.ru

This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Malinovskaya E. M., Savinova E. A., Sergeeva V. A., Ershova E. S., Proskurnina E. V., Kameneva L. V., Dolgikh O. A., Veiko N. N., Kraevaya O. A., Zhilenkov A. V., Troshin P. A., Kostyuk S. V. THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO // . 2022. pp. 325-333. DOI: https://doi.org/10.29039/rusjbpc.2022.0523. (Date of access 19.08.2026).

MLA

E. Malinovskaya, Savinova E. A., Sergeeva V. A., Ershova E. S., Proskurnina E. V., Kameneva L. V., Dolgikh O. A., Veiko N. N., Kraevaya O. A., Zhilenkov A. V., Troshin P. A., Kostyuk S. V.. "THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO." (2022): 325-333. DOI: https://doi.org/10.29039/rusjbpc.2022.0523.

APA

Malinovskaya E. M., Savinova E. A., Sergeeva V. A., Ershova E. S., Proskurnina E. V., Kameneva L. V., Dolgikh O. A., Veiko N. N., Kraevaya O. A., Zhilenkov A. V., Troshin P. A., Kostyuk S. V. (2022). THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO. , 325-333. https://doi.org/10.29039/rusjbpc.2022.0523

Chicago

E. Malinovskaya, Savinova E. A., Sergeeva V. A., Ershova E. S., Proskurnina E. V., Kameneva L. V., Dolgikh O. A., Veiko N. N., Kraevaya O. A., Zhilenkov A. V., Troshin P. A., Kostyuk S. V.. "THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO." , no. (2022): 325-333. https://doi.org/10.29039/rusjbpc.2022.0523.

WIKI

{{cite journal | author = Malinovskaya E. M., Savinova E. A., Sergeeva V. A., Ershova E. S., Proskurnina E. V., Kameneva L. V., Dolgikh O. A., Veiko N. N., Kraevaya O. A., Zhilenkov A. V., Troshin P. A., Kostyuk S. V. | title = THE EFFECT OF WATER-SOLUBLE DERIVATIVES OF FULLENE C60 ON THE LEVEL OF OXIDATIVE STRESS AND ON THE TRANSCRIPTIONAL ACTIVITY OF GENES REGULATING PRO- AND ANTIOXIDANT ACTIVITY OF HUMAN EMBRYONIC FIBROBLAST CELLS (HELFs) IN VITRO | journal = | year = 2022 | pages = 325-333 | doi = 10.29039/rusjbpc.2022.0523 }}

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