THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY

A. Soloveva

doi:10.29039/rusjbpc.2022.0542

UDC

577

THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY

Published in Russian Journal of Biological Physics and Chemisrty · Pages 446–450 · Rubric: MEDICAL BIOPHYSICS AND BIOPHYSICAL CHEMISTRY

DOI: https://doi.org/10.29039/rusjbpc.2022.0542

Received: 20.09.2022 Accepted: 20.09.2022 Published: 28.09.2022

Authors

A. Soloveva

Privolzhsky Research Medical University Russian Federation · Nizhny Novgorod

ABSTRACT

Combined thermal trauma (CTT), including thermal inhalation exposure and burns of the skin, is often found in victims entering burn centers. The aim of the study was to study the peculiarities of regulation of antioxidant protection enzymes in the blood during experimental CTT under the influence of NO. The experiment was carried out on 30 white male rats of the Wistar line. The animals were divided into 3 groups of equal numbers: 1 – intact rats; 2 – control – animals with CTT; 3 – experimental – animals with CTT who received inhalation NO. Rats under anesthesia were subjected to contact burn (20% of the body surface) and thermal inhalation exposure. The animals were removed from the experiment after CTT for 10 days. The activity of superoxide dimsutase (SOD), catalase, glutathione reductase (GR) and glucose-6-phosphate dehydrogenase (Gl-6-fDh) was determined in red blood cells. Inhalation-external exposure to NO during CTT was carried out daily for 10 days for 10 minutes. Kinetic parameters of the enzymatic reaction were calculated. Statistical processing of the results was carried out using Statistica 6.0 (Statsoft Inc., USA). It was shown that against the background of a decrease in the activity of antioxidant enzymes in the blood during CTT, the greatest inhibition was noted for catalase. The kinetic method of analysis for the first time established the nature of enzyme inhibition in CTT: for SOD and catalase in erythrocytes – two-parametrically consistent, for GR and Gl-6–fDh - catalytic. Inhalations of NO during CTT activated SOD, catalase, GR and Gl-6-fDh.

KEYWORDS

combined thermal injury, nitric oxide, antioxidant protection enzymes

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This work is licensed under Creative Commons Attribution 4.0 International Creative Commons

GOST

Soloveva A. G. THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY // . 2022. pp. 446-450. DOI: https://doi.org/10.29039/rusjbpc.2022.0542. (Date of access 19.08.2026).

MLA

A. Soloveva. "THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY." (2022): 446-450. DOI: https://doi.org/10.29039/rusjbpc.2022.0542.

APA

Soloveva A. G. (2022). THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY. , 446-450. https://doi.org/10.29039/rusjbpc.2022.0542

Chicago

A. Soloveva. "THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY." , no. (2022): 446-450. https://doi.org/10.29039/rusjbpc.2022.0542.

WIKI

{{cite journal | author = Soloveva A. G. | title = THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY | journal = | year = 2022 | pages = 446-450 | doi = 10.29039/rusjbpc.2022.0542 }}

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THE INFLUENCE OF NITRIC OXIDE ON KINETIC PROPERTIES OF ANTIOXIDANT PROTECTION ENZYMES IN THE BLOOD DURING EXPERIMENTAL THERMAL INJURY

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