UDC 577

BIOPHYSICAL PRINCIPLES IN HUMAN GAMETES CRYOPRESERVATION: CRYOPROTECTANTS MODIFIED BY EGG YOLK

Published in Russian Journal of Biological Physics and Chemisrty · Volume 1, Issue 1 · Pages 70–73 · Rubric: General biophysics
Received: 20.06.2016 Accepted: 20.06.2016 Published: 25.06.2016 Language of publication: RUS
Cryopreservation of human gametes is one of the most important methods of IVF allowing to save functional gametes prior to the treatment which might threaten male fertility. However, fertilizing capacity of spermatozoa after cryopreservation decreases by 30-70 % on average, explaining the relevance of search for new substances and protocols possessing improved cryoprotective properties. In this work we suggested modification of cryoprotectant by addition of specifically prepared egg yolk emulsion. Such modification showed positive influence on safety of fertile spermatozoa, - increasing the mean number of normal motile gametes after cryopreservation by 20 % and decreasing morphological defectiveness. The emulsion was tested for cytotoxicity and compared with commercially available cryoprotectant containing egg yolk.
gametes, cryoprotectant, spermatozoa, IVF, cryopreservation
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