Perspectives of using fluorescence of chlorophyll methods for the assessment of toxic effects of nanomaterials on phytoplankton is analyzed. Measuring of light dependence curves of fluorescence allows changes in energy storage in photosynthetic processes in algal cells to be detected at early stages of phytoplankton exposure to modern nanomaterials.
nanomaterials, phytoplankton, chlorophyll fluorescence, photosynthesis, ecology
1. Falkowski P.G., Raven J.A. Aquatic photosynthesis. Malden: Blackwell Science, 1997, 375 p.
2. Matorin D.N., Osipov V.A., Yakovleva O.V., Pogosyan S.I. Assessment of the state of plants and algae based on chlorophyll fluorescence. Guidance manual. Moscow: MSU, Max Press, 2010, 117 p.
3. Antal T.K., Venediktov P.S., Matorin D.N., Ostrowska M., Wozniak B., Rubin A.B. Measurement of phytoplankton photosynthesis rate using a pump-and-probe fluorometer. Oceanologia, 2001, vol. 43, no. 3, pp. 291-313.
4. Antal T. K., Matorin D.N., Ilyash L.V., Volgusheva A.A., Osipov V.A., Konyuhov I.V., Krendeleva T.E., Rubin A.B. Probing of photosynthetic reactions in four phytoplanktonic algae with a PEA fluorometer. Photosynthesis Res., 2009, vol. 102, pp. 67-76.
5. Antal T.K., Osipov V.A., Matorin D.N., Rubin A.B. Membrane potential is involved in regulation of photosynthetic electron transport in the marine diatom Thalassiosira weissflogii. Journal of Photochemistry and Photobiology: Biology, 2011, vol. 102, pp. 169-173.
6. Matorin D.N., Antal T.K., Ostrowska M., Rubin A.B., Ficek D. Chlorophyll fluorometry as a method for studying light absorption by photosynthetic pigments in marine algae. Oceanologia, 2004, vol. 46, no. 4, pp. 519-531.
7. Matorin D.N., Karateeva A.V., Osipov V.A., Lukashev E.P., Seifullina N.Kh., Rubin A.B. Influence of Carbon Nanotubes on Chlorophyll Fluorescence Parameters of Green Algae Chlamydomonas reinhardtii. Nanotechnologies in Russia, 2010, vol. 5, no. 5-6, pp. 321-328.
8. Schreiber U. Pulse-Amplitude (PAM) fluorometry and saturation pulse method. In: Chlorophyll fluorescence: A signature of Photosynthesis. Papageorgiou G. and Govindjee (eds.), Springer, Dordrecht, Netherlands, 2004, pp. 279-319.
9. Matorin D.N., Venediktov P.S., Konev Yu.V., Rubin A.B. Application of a doublee-flash, impulse, submersible fluorimeter in the determination of photosynthetic activity of natural phytoplankton. Doklady Russ. Acad. Sci., Earth Sci. Sect., 1996, vol. 350, no. 7, pp. 1159-1161.
10. Matorin D.N., Rubin A.B. Chlorophyll Fluorescence in Algae and Higher Plants. Moscow-Izhevsk: IKI- RKhD, 2012.
11. Wang J., Qiang Hu. Old bottle with new wine: algal indicators for nanotechnology-based toxicology. Electronic Journal of Biology, 2005, vol. 1, no. 1, pp. 9-13.
12. Terekhova V.A., Vavilova V.M., Fedoseeva E.W., Karateeva A.V., Matorin D.N., Gubarevich T.M. Detoxicating effect of humic preparations when used in combination with carbon nanoparticlesю Proceedings of V All- Russian Conference «Humic substances in biosphere». Part 1 - SPb.: Publishing house SPBGU, 2010, pp. 462-469.
13. Herlory O., Richard P. Blanchard G. F. Methodology of light response curves: application of chlorophyll fluorescence to microphytobenthic biofilms. Mar Biol., 2007, vol. 153, pp. 91-101.
