МИКРОСЕПАРАЦИОННАЯ ЯЧЕЙКА ДЛЯ ВЫДЕЛЕНИЯ ЦЕЛЕВОГО БЕЛКА МЕТОДОМ ЭЛЕКТРОФОРЕЗА В СВОБОДНОМ ПОТОКЕ
Аннотация и ключевые слова
Аннотация (русский):
Предложена конструкция упрощенного сепарационного устройства и методики для выделения целевого белка из смеси методом зонного электрофореза в свободном потоке. Эффективность устройства была продемонстрирована теоретически и экспериментально на примере сепарации белков гемоглобина и сывороточного альбумин человека. Предложенный метод сепарации отличается простотой использования и хорошей производительностью.

Ключевые слова:
микросепарационная ячейка, электрофорез
Текст
Текст произведения (PDF): Читать Скачать
Список литературы

1. Hoffmann P., Ji H., Moritz R.L. Continuous free-flow electrophoresis separation of cytosolic proteins from the human colon carcinoma cell line LIM 1215: a non-two-dimensional gel electrophoresis-based proteome analysis strategy. Proteomics, 2001, vol. 1, pp. 807-818.

2. Maida R., Krieger J., Gebauer T. Three pheromone-binding proteins in olfactory sensilla ofthe two silkmoth species Antheraea polyphemus and Antheraea pernyi. Eur. J. Biochem., 2000, vol. 267, pp. 2899-2908.

3. Wang Y., Hancock W.S., Weber G. Free flow electrophoresis coupled with liquid chromatography-mass spectrometry for a proteomic study often human cell line. J. Chromatogr., 2004, vol. 1053(A), pp. 269-278.

4. Kessler R., Manz H.J., Székely G. Use of free-flow electrophoresis for the purification of components separated by ion-pair chromatography. J. Chromatogr., 1989, vol. 469, pp. 444-447.

5. Hansen E., Hannig K. Antigen-Specific Electrophoretic Cell Separation (ASECS): isolation of human T and B lymphocyte subpopulations by free-flow electrophoresis after reaction with antibodies. J. Immunol. Methods, 1982, vol. 51, pp. 197-208.

6. Eubel H., Lee C.P., Kuo J. Technical Advance: free-flow electrophoresis for purification of plant mitochondria by surface charge. Plant J., 2007, vol. 52, pp. 583-594.

7. Zhang C., Manz A. High-speed free-flow electrophoresis on chip. Anal. Chem., 2003, vol. 75, pp. 5759-5766.

8. Nissum M., Shehab M. A. Functional and Complementary Phosphorylation State Attributes of Human Insulin-like Growth Factor-Binding Protein-1 (IGFBP-1) Isoforms Resolved by Free Flow Electrophoresis. Mol. Cell. Proteomics, 2009, vol. 8, pp. 1424-1435.

9. Islinger M., Li K. W., Maarten Loos M., Liebler S., Angermuller S. Peroxisomes from the heavy mitochondrial fraction: isolation by zonal free flow electrophoresis and quantitative mass spectrometrical characterization. J. Proteome Res., 2010, vol. 9, pp. 113-124.

10. Ouvry-Patat S. A., Torres M. P., Quek H. H. Free-flow electrophoresis for top-down proteomics by Fourier transform ion cyclotron resonance mass spectrometry. Proteomics, 2008, vol. 8, pp. 2798-2808.

11. Moritz R., Ji H., Schutz F. A Proteome Strategy for Fractionating Proteins and Peptides Using Continuous Free-Flow Electrophoresis Coupled Off-Line to Reversed-Phase High-Performance Liquid Chromatography. Anal. Chem., 2004, vol. 76, pp. 4811-4824.

12. Malmstrom J., Lee H., Nesvizhskii A., Shteynberg D.J. Advances in proteomic workflows for systems biology. Proteome Res., 2006, vol. 5, pp. 2241-2249.

13. Raymond D.E., Manz A., Widmer H.M. Continuous sample pretreatment using a Free-Flow electrophoresis device integrated onto a silicon chip. Anal. Chem., 1994, vol. 66, pp. 2858-2865.

14. Fonslow B.R., Barocas V.H., Bowser M.T. Using channel depth to isolate and control flow in a micro Free-Flow electrophoresis device. Anal. Chem., 2006, vol. 78, pp. 5369-5374.

15. Geng J., Shao J., Yang J. Reassemblable quasi-chip free-flow electrophoresis with simple heating dispersion for rapid micropreparation of trypsin in crude porcine pancreatin. Electrophoresis, 2011, vol. 32, pp. 3248-3256.

16. Kasiˇcka V. From micro to macro: conversion of capillary electrophoretic separations of biomolecules and bioparticles to preparative free-flow electrophoresis scale. Electrophoresis, 2009, vol. 30, pp. 40-52.

17. Raymond D., Manz A., Widmer H.M. Continuous separation of high molecular weight compounds using a microliter volume free-flow electrophoresis microstructure. Anal. Chem., 1996, vol. 68, pp. 2515-2522.

18. Kwon J.S., Bowser M.T. Micro Free-Flow Electrophoresis. Encyclopedia of Nanotechnology, 2015, vol. 28, pp. 1-15.

19. Kobayashi H., Shimamura K., Akaida T. Free-flow electrophoresis in a microfabricated chamber with a micromodule fraction separator: continuous separation of proteins. J. Chromatogr., 2003, vol. 990 (A), pp. 169-178.

20. Zhang C.-X., Manz A. High-speed free-flow electrophoresis on chip. Anal. Chem., 2003, vol.75. pp. 5759-5766.

21. Fonslow B.R., Bowser M.T. Free-flow electrophoresis on an anodic bonded glass microchip. Anal. Chem., 2005, vol. 77, pp. 5706-5710.

22. Medhat A. Al-Ghobashy Electrophoretic behavior of charge regulated zwitter ionic buffers in covalently and dynamically coated fused silica capillaries. Bulletin of Faculty of Pharmacy, Cairo University, 2014, vol. 52, pp. 71-78.


Войти или Создать
* Забыли пароль?