Карбоксиангидраза представляет собой однодоменный глобулярный белок, в нативном состоянии которого преобладает β-структура. В данной работе проведено исследование равновесного разворачивания апоформы карбоксиангидразы понижением рН. Показано, что денатурация белка кислым рН представляет собой переход между тремя состояниями N → I1→ I2. Добавление мочевины к I2 приводит к его разворачиванию до развернутого состояния I2 → U. С помощью аппроксимации переходов равновесного разворачивания белка понижением рН и мочевиной удалось провести оценку стабильностей нативного и двух промежуточных состояний карбоксиангидразы. Описанный подход может быть использован в дальнейшем для исследования влияния аминокислотных замен на сворачивание данного белка. Также в работе были исследованы структурные особенности конформационных состояний карбоксиангидразы, полученные результаты позволили сделать вывод, что состояние I1 находится в составе ассоциатов и содержит ненативные α-спиральные участки.
карбоксиангидраза, сворачивание белка, промежуточное состояние, равновесное разворачивание, круговой дихроизм
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