Saint Petersburg, St. Petersburg, Russian Federation
Using Fura-2AM microfluorimetry the involvement of lipid rafts in the effect of immunomodulator glutoxim on intracellular Ca2+ concentration in rat peritoneal macrophages was investigated. Glutoxim induces biphasic Ca2+-response associated with Ca2+ release from intracellular Ca2+ stores and subsequent store-dependent Ca2+ entry in macrophages. We have shown for the first time that cell preincubation with methyl- β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, significantly decreases both phases of Ca2+-responses induced by glutoxim in peritoneal macrophages. The results suggest that intact rafts are necessary for signaling cascade triggered by glutoxim and leading to intracellular Ca2+-concentration increase in macrophages.
peritoneal macrophages, glutoxim, lipid rafts
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