Mesenchymal stromal cells (MSCs) represent a heterogeneous population of proliferating non- differentiated cells that capable of originating a variety of mesenchymal cell lineages. Despite tremendous progress in MSC biology, current knowledge on receptor and signaling systems of MSCs is mediocre. Here we showed that ATP and other purinergic agonists, including АDР, UTP, UDP stimulate Са2+ signaling in a subpopulation of MSCs derived from the human adipose tissue. The rank of agonist efficacies and negligible influence of extracellular Ca2+ on cellular responses argued that purinergic transduction in MSCs largely involved P2Y receptors. By the RT-PCR analysis revealed expression of P2Y1, P2Y2, P2Y4, P2Y6 purinoreceptors in MSC. In individual MSC these receptors function in different combinations, but the main role in generation Са2+-responses to purinergic agonists play P2Y1, P2Y2, P2Y4 receptors.
mesenchymal stromal cells, purinoreceptors
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