BIOPHYSICAL PRINCIPLES IN HUMAN GAMETES CRYOPRESERVATION: CRYOPROTECTANTS MODIFIED BY EGG YOLK
Rubrics: GENERAL BIOPHYSICS
Authors:
1.
Lomonosov Moscow State University
2.
Lomonosov Moscow State University
3.
Lomonosov Moscow State University; IVF Clinic AltraVita
4.
Lomonosov Moscow State University
5.
Lomonosov Moscow State University
(Department of Biophysics, Faculty of Physics, Head of the Department)
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
6.
IVF Clinic AltraVita
7.
IVF Clinic AltraVita
Type:
Article
Pages:
from 70
to 73
Status:
Published
Received:
20.06.2016
Accepted:
20.06.2016
Published:
25.06.2016
Subject area:
UDK 577 Материальные основы жизни. Биохимия. Молекулярная биология.Биофизика
Language:
Russian
Keywords:
gametes, cryoprotectant, spermatozoa, IVF, cryopreservation
Abstract (English):
Cryopreservation of human gametes is one of the most important methods of IVF allowing to save functional gametes prior to the treatment which might threaten male fertility. However, fertilizing capacity of spermatozoa after cryopreservation decreases by 30-70 % on average, explaining the relevance of search for new substances and protocols possessing improved cryoprotective properties. In this work we suggested modification of cryoprotectant by addition of specifically prepared egg yolk emulsion. Such modification showed positive influence on safety of fertile spermatozoa, - increasing the mean number of normal motile gametes after cryopreservation by 20 % and decreasing morphological defectiveness. The emulsion was tested for cytotoxicity and compared with commercially available cryoprotectant containing egg yolk.
Cryopreservation of human gametes is one of the most important methods of IVF allowing to save functional gametes prior to the treatment which might threaten male fertility. However, fertilizing capacity of spermatozoa after cryopreservation decreases by 30-70 % on average, explaining the relevance of search for new substances and protocols possessing improved cryoprotective properties. In this work we suggested modification of cryoprotectant by addition of specifically prepared egg yolk emulsion. Such modification showed positive influence on safety of fertile spermatozoa, - increasing the mean number of normal motile gametes after cryopreservation by 20 % and decreasing morphological defectiveness. The emulsion was tested for cytotoxicity and compared with commercially available cryoprotectant containing egg yolk.
Keywords:
gametes, cryoprotectant, spermatozoa, IVF, cryopreservation
gametes, cryoprotectant, spermatozoa, IVF, cryopreservation
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