Donetsk, Russian Federation
Now the mechanism of complex mutations formation that is called cyclobutane pyrimidine dimers has not been yet explained. Complex frameshifts are frameshifts with an adjacent base substitution. The author has proposed and developed polymerase-tautomeric model of ultraviolet mutagenesis. The mechanism of targeted complex insertions formation that is called cis-syn cyclobutane thymine dimers is proposed. It is cconsidered error- prone or SOS-synthesis of double-stranded DNA containing in one of its strands cis-syn cyclobutane thymine dimers TT1*, TT2 and TT5*. Cis- syn cyclobutane thymine dimers TT1*, TT2 and TT5* have DNA bases in rare tautomeric forms. Cis-syn cyclobutane thymine dimers TT2* leads to a frameshift mutations, that is to insert one or more nucleotides. Cis-syn cyclobutane thymine dimers TT1* and TT5* cause substitution mutations formation. Thus, due to the formation of DNA insertion portion is extended and due to the formation of several base substitution mutations changing its nucleotide composition. As a result of the DNA portion of a certain length and nucleotide composition of a certain portion is replaced by a different length and a different nucleotide composition. Targeted complex mutation is appeared.
ultraviolet mutagenesis, polymerase-tautomeric model, targeted complex mutations, targeted complex insertions, error-prone replication, SOS replication, rare tautomeric forms of DNA bases, cis-syn thymine cyclobutane dimers
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