STUDY OF THE DISTRIBUTION OF TRANSPOSABLE ELEMENTS IN THE GENOME LITTORINA SAXATILIS
Abstract and keywords
Abstract (English):
In this work it was studied the distribution of transposable elements (TE) in the genome of the marine gastropod Littorina saxatilis . We investigated the nucleotide sequence of a length of about 360 thousand bp. Genome analysis L. saxatilis has revealed the repeats that are similar to the retrotransposons and DNA-transposons. The number of detected retrotransposons was higher than the amount of DNA-transposons in two and a half times (77 and 30, respectively). This is consistent with the fact that the replicative way to move contributes to the accumulation of the TE in the genome. Among the most widely represented retrotransposons were members of families of Copia , Gypsy , CR1 and DIRS . Among the DNA transposons were the most numerous family of Mariner , hAT and Sola . Further, we going to study the structure of the detected repetition to determine which ones are potentially functional TE.

Keywords:
Littorina saxatils, DNA, transposable elements, stress induction, insertional mutagenesis, Littorina saxatilis
Text
Publication text (PDF): Read Download
References

1. Yurchenko N.N., Kovalenko L.V., Zaharov I.K. Mobil'nye geneticheskie elementy: nestabil'nost' genov i genomov. Vavilovskiy zhurnal genetiki i selekcii, 2011, № 15 (2), s. 261-270. [Yurchenko N.N., Kovalenko L.V., Zakharov I.K. Transposable elements: instability of genes and genomes. Vavilovskyi zhurnal genetiki i selektsii, 2011, vol. 15 (2), pp. 261-270. (In Russ.)]

2. Chow K.C., Tung W.L. Magnetic field exposure stimulates transposition through the induction of DnaK/J synthesis. Biochem Biophys Res Commun., 2000, vol. 270 (3), pp. 745-748.

3. Bubenschikova E.V., Antonenko O.V., Vasil'eva L.V., Ratner V.A. Indukciya transpoziciy MGE 412 razdel'no teplovym i holodovym shokom v spermatogeneze u samcov drozofily. Genetika, 2002, № 38 (1), s. 46-55. [Bubenshchikova E.V., Antonenko O.V., Vasil'eva L.A., Ratner V.A. Induction of MGE 412 transposition individually by heat and cold shock in spermatogenesis in Drosophila males. Genetika, 2002, vol. 38 (1), pp. 46-55. (In Russ.)]

4. Del Re B., Garoia F., Mesirca P., Agostini C., Bersani F., Giorgi G. Extremely low frequency magnetic fields affect transposition activity in Escherichia coli. Radiat Environ Biophys., 2003, vol. 42 (2), pp. 113-118.

5. Zaharenko L.P., Kovalenko L.V., Perepelkina M.P., Zaharov I.K. Vliyanie γ-radiacii na indukciyu transpoziciy hobo-elementa u Drosophila melanogaster. Genetika, 2006, № 42 (6), s. 763-767. [Zakharenko L.P., Kovalenko L.V., Perepelkina M.P., Zakharov I.K. The effect of gamma-radiation on induction of the hobo element transposition in Drosophila melanogaster. Genetika, 2006, vol. 42 (6), pp. 763-767. (In Russ.)]

6. Piacentini L., Fanti L., Specchia V., Bozzetti M.P., Berloco M., Palumbo G., Pimpinelli S. Transposons, environmental changes, and heritable induced phenotypic variability. Chromosoma, 2014, vol. 123, pp. 345-354.

7. Rebollo R., Horard B., Hubert B., Vieira C. Jumping genes and epigenetics: towards new species. Gene, 2010, vol. 454, pp. 1-7.

8. McClintock B. Controlling elements and the gene. Cold Spring Harbor Sympos. Quant. Biol., 1956, vol. 21, p. 197.

9. Mobile DNA II. Edited by Craig N., Craigie R., Gellert M., Lambowitz A. Washington DC: American Society of Microbiology Press, 2002.

10. de Koning A.P., Gu W., Castoe T.A., Batzer M.A., Pollock D.D. Repetitive elements may comprise over two- thirds of the human genome. PLoS Genet., 2011, vol. 7 (12), p. e1002384.

11. Sergeeva E.M., Salina E.A. Mobil'nye elementy i evolyuciya genoma rasteniy. Vavilovskiy zhurnal genetiki i selekcii, 2011, № 15 (2), s. 382-398. [Sergeeva E.M., Salina E.A. Transposable elements and plant genome evolution. Vavilovskyi zhurnal genetiki i selektsii, 2011, vol. 15 (2), pp. 382-398. (In Russ.)]

12. Guo B., Zou M., Gan X., He S. Genome size evolution in pufferfish: an insight from BAC clone-based Diodon holocanthus genome sequencing. BMC Genomics, 2010, vol. 11, p. 396.

13. Kapitonov V.V., Jurka J. A universal classification of eukaryotic transposable elements implemented in Repbase. Nat Rev Genet., 2008, vol. 9 (5), pp. 411-412.

14. Pearce S.R., Pich U., Harrison G., Flavell A.J., Heslop-Harrison J.S., Schubert I., Kumar A. The Ty1-copia group retrotransposons of Allium cepa are distributed throughout the chromosomes but are enriched in the terminal heterochromatin. Chromosome Res., 1996, vol. 4 (5), pp. 357-364.

15. Kumar A., Bennetzen J.L. Plant retrotransposons. Annu Rev Genet., 1999, vol. 33, pp. 479-532.

16. Georgiev G.P. Mobile genetic elements in animal cells and their biological significance. Eur J Biochem., 1984, vol. 145 (2), pp. 203-220.

17. Sormacheva N.D., Blinov A.G. LTR retrotranspozony rasteniy. Vavilovskiy zhurnal genetiki i selekcii, 2011, № 15 (2), s. 351-381. [Sormacheva I.D., Blinov A.G. LTR retrotransposons in plants. Vavilovskyi zhurnal genetiki i selektsii, 2011, vol. 15 (2), pp. 351-381. (In Russ.)]

18. Cordaux R., Udit S., Batzer M.A., Feschotte C. Birth of a chimeric primate gene by capture of the transposase gene from a mobile element. Proc Natl Acad Sci USA, 2006, vol. 103 (21), pp. 8101-8106.

19. Jurka J. Conserved eukaryotic transposable elements and the evolution of gene regulation. Cell Mol Life Sci., 2008, vol. 65, pp. 201-204.

20. Lim J.K., Simmons M.J. Gross chromosome rearrangements mediated by transposable elements in Drosophila melanogaster. Bioessays, 1994, vol. 16 (4), pp. 269-275.

21. Rebollo R., Romanish M.T., Mager D.L. Transposable elements: an abundant and natural source of regulatory sequences for host genes. Annu Rev Genet., 2012, vol. 46, pp. 21-42.

22. Kohany O., Gentles A.J., Hankus L., Jurka J. Annotation, submission and screening of repetitive elements in Repbase: RepbaseSubmitter and Censor. BMC Bioinformatics, 2006, vol. 25 (7), p. 474

23. Jurka J. Repeats in genomic DNA: mining and meaning. Curr. Opin. Struct. Biol., 1998, vol. 8, pp. 333-337.

24. Llorens C., Muñoz-Pomer A., Bernad L., Botella H., Moya A. Network dynamics of eukaryotic LTR retroelements beyond phylogenetic trees. Biol Direct., 2009, vol. 4, p. 41.


Login or Create
* Forgot password?