Vladivostok, Vladivostok, Russian Federation
The comparative analysis of phycobiliproteins and sulfated polysaccharides isolated by successive extraction by some various methods from red algae Ahnfeltiopsis flabelliformis and Mastocarpus pacificus (Phyllophoraceae) Sea of Japan was performed. It was found that efficient extraction of phycobiliproteins was by means of 1.5 % sodium nitrate solution. The method of spectral analysis revealed that the major pigment in both algae was phycoerythrin with its content being 3-folds higher in A. flabelliformis than in M. pacificus . It was shown that flabelliformis produced greater amount of polysaccharides by comparison to M. pacificus . According to IR spectroscopy, polysaccharides from A. flabelliformis and M. pacificus had a hybrid structure and represented kappa/iota-carrageenans. It was shown by dynamic light scattering (DLS) and electrokinetic measurements that the isolated polysaccharides were characterized by a large polydispersity and a high value of negative surface charge.
Ahnfeltiopsis flabelliformis, Mastocarpus pacificus, phycobiliproteins, sulfated polysaccharides, IR-spectroscopy, Ahnfeltiopsis flabelliformis, Mastocarpus pacificus
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