He interaction of recombinant (R-OmpF) and chimeric OmpF porin from outer membrane of Yersinia pseudotuberculosis (Ch-OmpF) with negatively charged liposomes was studied. It was shown that the porin spatial structure in Ch-OmpF supported by DsbC protein-chaperone in the presence of lipid bilayer can take a functional activity conformation. The effectiveness of pore formation depended on the composition of the liposomal membrane and the presence of lipopolysaccharide.
porin, pore-forming activity, liposomes, lipopolysaccharide
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