Due to the active progress of cell transplantology as branch of regenerative medicine, development of techniques for precise cell targeting in vivo has a great relevance. Application of magnetic nanoparticles as cell markers becoming a rather popular tool for obtaining of controlled cell products. As a result of magnetic labeling, the marked cell acquires the possibility of being controlled by means of a magnetic field. In the presented work the magnetic properties of human fetal fibroblasts (HFF) obtained after incubation with Fe3O4 nanoparticles at a short exposure time were investigated. Such characteristics of the modified cells as viability, intracellular iron content and magnetic susceptibility (χ) were defined. A computer model of cell capture in the conditions of the blood channel in the presence of an external magnetic field based on calculated experimental data was constructed. The results of the simulation indicate that the capture efficiency of the labeled cells obtained by the described procedure is linearly dependent on the value of χ and can reach about 60 %.
magnetic nanoparticles, magnetic field, magnetic susceptibility, human fetal fibroblasts
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