APPLICATION OF ION-EXCHANGE CHROMATOGRAPHY IN ASSESSING THE BIOLOGICAL VALUE OF SHEEP MILK PROTEINS
Abstract and keywords
Abstract (English):
Assessment of the biological value of livestock products is an actual trend of modern science and agriculture practice, in addition, a quantitative analysis of the amino acid composition of proteins plays an important role in biochemical, pharmaceutical and biomedical research. This paper is devoted to the use of ion-exchange chromatography with post-colum derivatization by ninhydrin in the study of the amino acid composition and evaluation of the biological value of sheep milk proteins. The amino acid composition of the milk proteins of the Romanov sheep breed obtained on the 20th day of lactation is presented, the amino acid score of these proteins are calculated, the ratio of essential and other amino acids is calculated, biological value of milk proteins compared with the "ideal" protein (FAO WHO) is estimated. It was found that in milk proteins of Romanov sheep obtained on the 20th day of lactation, threonine is a limiting amino acid.

Keywords:
ion-exchange chromatography, post-column derivatization, ninhydrin, amino acid score, sheep milk
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