Influence of 100 µM aluminum ions on amyloids fibril growth from lysozyme was studied. Using fluorescent probe thioflavin T it was shown that addition aluminum ions to growth medium of amyloid fibrils from lysozyme leads to increasing fluorescent intensity of applied probe. This suggests that aluminum ions stimulate formation of amyloid fibrils. However, applying another fluorescent probe - 1-anilinonaphthalene 8-sulfonate (ANS) we couldn’t find structural differences between amyloid fibril with and without aluminum chloride in the environment. Also we studied own fluorescent of tyrosine and tryptophan of amyloid fibrils from lysozyme ad their complexes with aluminum ions. It was shown that 100 µM aluminum ions lead to decreasing intrinsic fluorescence in compare with amyloid fibrils only from lysozyme.
amyloid structures, lysozyme, aluminum chloride, fluorescent probes
1. Woodard D., Bell D., Tipton D., Durrance S., Cole L., Li B., Xu S. Gel formation in protein amyloid aggregation: A physical mechanism for cytotoxicity. PLOS ONE, 2014, vol. 9, no. 4, pp. 1-8.
2. Zawilla N.H., Taha F.M., Kishk N.A., Farahat S.A., Farghaly M., Hussein M. Occupational exposure to aluminum and its amyloidogenic link with cognitive functions. Journal of Inorganic Biochemistry, 2014, no. 139, pp. 57-64.
3. Kayed R., Head E., Thompson J.L., McIntire T.M., Milton S.C., Cotman C.W., Glabe C.G. Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science, 2003, vol. 300, pp. 486-489.
4. Hosokawa S., Nishitani H., Nishio T., Imai T., Tomita Y., Tomoyoshi T., Sawanishi K., Yoshida O. Aluminum transport and Dialysance during Haemodialysis. International Urology and Nephrology, 1988, vol. 20, no. 1, pp. 89-100.
5. Aremu D.A., Meshitsuka S. Accumulation of aluminum by primary cultured astrocytes from aluminum amino acid complex and its apoptotic effect. Brain Res., 2005, vol. 1031, pp. 284-296.
6. Yokel R.A., Rhineheimer S.S., Sharma P., Elmore D., McNamara P.J. Entry, half-life and desferrioxamine-accelerated clearance of brain aluminum after a single (26) Al exposure. Toxicol. Sci., 2001, vol. 64, pp. 77-82.
7. Andrási E., Páli N., Molnár Z., Kösel S. Brain aluminum, magnesium and phosphorus contents of control and Alzheimer-diseased patients. J. Alzheimers Dis., 2005, vol. 7, pp. 273-284.
8. Yokel A.R, Golub M.S. Research issues in aluminum toxicity. Washington, DC: Taylor and Francis, 1997, 116 p.
9. Sanei H., Goodarzi F., Flier-Keller E.V. Historical variation of elements with respect to different geochemical fractions in recent sediments from Pigeon Lake, Alberta, Canada. J. Environ. Monit., 2001, vol. 3. pp. 27-36.
10. Lévesque L., Mizzen C.A., McLachlan D.R., Fraser P.E. Ligand specific effects on aluminum incorporation and toxicity in neurons and astrocytes. Brain Res., 2000, vol. 877, pp. 191-202.
11. Zafar T.A., Uppal A. Aluminum intake and it’s toxic effects on health. International J. of Agriculture and Biology, 1999, vol. 1-3, pp. 188-191.
12. Bolognsi B., Kumita J.R., Barros T.P., Esbjorner E.K., Luheshi L.M., Crowther D.C., Wilson M.R., Dobson C.D., Favrin G., Yerbury J.J. ANS binding reveals common features of cytotoxic amyloid species. ACS Chemical biology, 2010, vol. 5, no 8, pp. 735-740.
13. Chen W-T., Liao Y-H., Yu H-M., Cheng I.H., Chen Y.R. Distinct effects of Zn2+, Cu2+, Fe3+ and Al3+ on amyloid-β stability, oligomerization and aggregation: Amyloid-β destabilization promotes annular protofibril formation. The journal of biological chemistry, 2011, vol. 286, pp. 9646-9656.
14. Morozova-Roche L.A., Zurdo J., Spencer A., Noppe W., Receveur V., Archer D.B., Joniau M., Dobson C.M. Amyloid fibril formation and seeding by wild-type human lysozyme and its disease-related mutational variants. Journal of Structural Biology, 2000, no. 130, pp. 339-351.
