Mesenchymal stromal cells (MSCs) are a heterogeneous population of proliferating undifferentiated cells that are capable of differentiating into cells of at least bone, cartilaginous and adipose lines. Despite significant progress in the study of MSC, the current understanding of their receptor and signal systems is very limited. In this paper, it is shown that adenosine stimulates Ca2+ signaling in MSC isolated from human adipose tissue. The threshold concentration of adenosine was determined, dose-dependences of response magnitude and lag-period were characterized. The data of the inhibitor analysis as well as insignificant influence of external Ca2+ on adenosine responses suggested that in the MSC, adenosine receptors are coupled by the phosphoinositide cascade mainly to Ca2+ release from Ca2+ store, while Ca2+ influx insignificantly contributes to Ca2+ signaling induced by adenosine.
mesenchymal stromal cells, adenosine receptors
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