N.N. Burdenko Voronezh State Medical University
Voronezh, Voronezh, Russian Federation
The physical, chemical and kinetic properties of cysteine proteinases - ficin and papain were studied. It was found that ficin is high active in the temperature range from 37 to 60 °C. The free ficin is completely inactivated at 70 °C, the enzyme sorbed on the chitosan retains about 50 % of the activity. The operating temperature range of soluble and immobilized papain is between 50 and 70 °C. The native enzyme is more incur to thermal inactivation than the enzyme sorbed on chitosan at 80 °C. It is shown that the immobilization of ficin and papain on chitosan matrices promotes the expansion of their pH optimum to the alkaline region. The maximum rate of enzymatic reaction of native and immobilized cysteine proteases is observed at a substrate concentration of 0,04 mM. Sorption of biocatalysts led to a decrease in the rate of enzymatic catalysis, as well as to a change in the degree of affinity of the enzymes to the substrate.
ficin, papain, immobilization, physical and chemical properties of enzymes, chitosan
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