Tomsk, Tomsk, Russian Federation
The role of hydrogen sulfide (H2S) in ATP action on tone of rat aorta segments with intact endothelium was studied. ATP (1-1000 µM), a nonselective activator of P2X and P2Y receptors, relaxed vascular segments precontracted with agonist of α1-adrenergic receptors phenilephrine (PE, 10 µM). Activator of P2X receptors α,β-MeATP (100 µM) also relaxed segments precontracted with PE (10 µM). Activator of P2Y receptors UTP (1-100 µM) decreased and UTP (100-1000 µM) increased the amplitude of PE-induced contraction. Relaxing action of ATP was augmented by the donor of hydrogen sulfide NaHS (500 µM), but decreased in the presence of cystotionine-γ-lyase (CSE) DL-propargylglicine (10 mM). DL-propargylglicine (10 mM) increased the amplitude of constrictive action of UTP (100- 1000 µM), but decreased relaxing action of α,β-MeATP (100 µM). In conclusion, activation of P2X receptors decreases the amplitude of PE-induced contraction and activation of P2Y - increases it. Relaxing action of ATP in rat aorta segments with intact endothelium is mediated by CSE activation through P2X receptors.
ATP, hydrogen sulfide, purinergic receptors, smooth muscle cells
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