Donetsk, Russian Federation
A comparative study of the enzymes activity of thymidine, adenosine metabolism and the activity of antioxidant defense enzymes in tumors of different localizations was carried out. Aim: to evaluate the relevance of thymidine phosphorylase (TP), adenosine deaminase (ADA) in 2-deoxy-D-ribose (2-d-D-R-1-P) formation and their association with superoxide dismutase, glutathione peroxidase activity in tumors. We demonstrated that tumor TP activity is higher by 1,8 times (p = 0,002), ADA activity is higher by 1,7-1,9 times (p = 0,001) as compared to non-neoplastic tissues of resection margin. The positive correlation between the intensity of TP and ADA activity was shown (the index of Spearman’s rank correlation, i.e. ρ = 0,704; р < 0,001). So, tumors can increase the levels of 2-deoxy-D-ribose (2-d-D-R) and can stimulate of deoxythymidilate synthesis by the «salvage pathway» in one time. The nucleoside enzymes activity changes being accompanied by decrease of glutathione peroxidase activity in the tumors. Conclusion: It could be suggested that in cancers of different localizations the glycation by 2-d-D-R being associated with hydrogen peroxide formation. Here, we provide evidence on the impact and cooperation of glycative stress and oxidative stress in promoting human cancer progression.
enzymes, 2-deoxy-D-ribose, hydrogen peroxide, cancer
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