We studied the homology of amino acid sequences of glycolytic enzymes: exo-inulinases ADM21204.1, A.CAC44220.1, EHA22512.1, AHN08014.1, AGR40655.1 and BAC45010.1, endo-inulinases AAN64131.1, ABB59681.1, EHA19510.1, XP_748286 .1, ANY59682.1 and AA02437.1, β-fructosidases CAA04518.1 and CAA52620.1, fructan-1-exohydrolase CAC37922.1 and fructosyltransferase ADK46938.1. The highest amino acid sequences identity value among exo-inulinases was detected for the enzymes from A. awamori and A. ficuum (90.88%), the smallest is between the enzyme from A. niger with enzymes from B. licheniformis and G. stearothermophilus (28.29%). The highest identity value for endo-inulinases is detected between two forms of endo-inulinase from A. niger (ABB59681.1 and EHA19510.1) and amounts to 97.87%, which is also the highest indicator for all enzymes presented in the work, the smallest identity is between the enzymes from A. fumigatus and K. marxianus (27.89%). The smallest indicator of homology among all enzymes presented in the work was detected between exo-inulinase from A. niger and β-fructosidase from T. maritima and was equal to 20.45%. The data presented in the paper demonstrate a significant amino acid composition of carbohydrates, which indicates the wide possibilities of their use in various conditions of industrial hydrolysis of inulin.
glycoside-hydrolases, homology, amino acid sequences
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