The mechanisms of exposure to UV light in the dose range of 151-6040 J/m2 on trypsin and collagenase free and immobilized on a matrix of acid-soluble medium molecular weight (200 kDa) and high molecular weight (350 kDa) chitosans were studied. The enzymatic activity of molecules of free proteases under the influence of UV light is subject to change to a greater extent than in the immobilized state. Immobilization leads to an increase in the stability of heterogeneous biocatalysts with respect to UV irradiation compared to free enzymes, possibly due to the formation of an enzyme - carrier complex, proteins become more resistant to such a physical agent as UV light. The data presented in this paper testify to the photoprotective effect of the chitosan matrix, which may be due to the following reasons: trypsin and collagenase molecules interact with the chitosan matrix to form photoresistant complexes; Chitosan molecules screen active photoproducts of a free radical nature, preventing the photo-oxidation of a certain number of amino acids of these enzymes when exposed to UV light.
UV radiation, trypsin, collagenase, immobilization, chitosan
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