Institute of Botany ANAS
Baku, Azerbaijan
By using of microelectrode techniques, patterns of change in the potential (φm) and resistance (Rm) of the plasma membrane (PM) of Chara fragilis cells under the influence of Dandelion-Burdock (DBC) phytocomposition solutions were studied. It is important to emphasize that the results of the analysis of the potential distribution, resistance of the plasma membrane by the number of cells of Chara fragilis under the standard conditions is being presented for the first time. The Hogg method allows simultaneous measuring of electrophysiological parameters such as φm and Rm. The average value of these parameters were -183±4.9 mV and 9±1.2 Оhm·m2 under the standard conditions. For the action of low DBC concentrations (0.01 mg/ml) in the activation range of the KCOR was characterized by significant depolarization of PM. In this paper, the inhibition of ion transport through the PM is discussed from the point of view of oxidation, and hyperpolarization-changes in the physical state of its lipid phase.
Chara fragilis, membrane potential, membrane resistance, phytoprotectors, H+ - pumps, K+ - channels
1. Boev R.S. Veschestvo s citostaticheskoy i apoptozinduciruyuschey aktivnost'yu iz korney lopuha. Himiya v interesah ustoychivogo razvitiya, 2005, t. 13, s. 119-122. @@[Boev R.S. Substance with cytostatic activity from burdock roots. Chemistry for Sustainable Development, 2005, vol. 13, pp. 119-122. (In Russ.)]
2. Vasin M.V. Protivoluchevye lekarstvennye sredstva. M., 2010, 182 s. @@[Vasin M.V. Radiotherapy drugs. M., 2010, 182 p. (In Russ.)]
3. Demidchik V.V. Membrannye mehanizmy regulyacii aktivnosti ionov kal'ciya v citoplazme kletok vysshih rasteniy. Trudy BGU, 2012, t. 7, ch. 1, s. 99-105. @@[Demidchik V.V. Membrane mechanisms of regulation of the activity of calcium ions in the cytoplasm of cells of higher plants. Proceedings of BSU, 2012, vol. 7, part 1, pp. 99-105. (In Russ.)]
4. Lakin G.F. Biometriya. M.: Vysshaya shkola, 1990, 352 s. @@[Lakin G.F. 1990. Biometrics. Moscow: Higher School, 352 p. (In Russ.)]
5. Medvedev S.S. Kalcievaya signal'naya sistema rasteniy. Fiziologiya rasteniy, 2005, t. 52, № 1, s. 15-24. @@[Medvedev S.S. Calcium signaling system of plants. Plant Physiology, 2005, vol. 52, pp. 15-24. (In Russ.)]
6. Ahmad V.U., Yasmeen S., Ali Z., Khan M.A., Choudhary M.I., Akhtar F., Miana G.A., Zahid M. Taraxacin, a new guaianolide from Taraxacum wallichii. J. Nat. Prod., 2000, vol. 63, pp. 1010-1011.
7. Choi J.H., Shin K.M., Kim N.Y., Lee Y.S., Kim H.J., Park H.J., Lee K.T. Taraxinic acid, a hydrolysate of sesquiterpene lactone glycoside from the Taraxacum coreanum Nakai, induces the differentiation of human acute promyelocytic leukemia HL-60 cells. Biol. Pharm. Bull., 2002, vol. 25, pp. 1446-1450.
8. Demidchik V. Plant extracellular ATP signaling by PM NADPH oxidase and Ca2+channels. Plant journal, 2009, vol. 58, pp. 903-913.
9. Hasanova A., Najafaliyeva Sh., Aliyeva P., Musayev N. Chara Fragilis cells - a new object for electrophysiology research. Journ. of Baku Engineering University - Chemistry and Biology, 2017, vol. 1, no. 2, pp. 176-182.
10. Hogg J., Williams E.J., Jhonston R.I. A simplified method for measuring membrane resistances Nitella translucens. Biochim. et Biophys. Acta, 1968, vol. 150, p. 518.
11. Hogg J., Williams E.J., Jhonston R.I. Light intensity and the membrane parameters of Nitella translucens. Biochim. et Biophys. Acta, 1969, vol. 173, pp. 564-566.
12. Voccoli V., Tonazzini I., Signore G. Role of extracellular calcium and mitochondrial oxygen species in psychosine-induced oligoden-drocyte cell death. Cell Death and Disease, 2014, vol. 5, pp. 1-12.
13. Yang D.S., Whang W.K., Kim I.H. The constituents of Taraxacum hallaisanensis roots. Arch. Pharmacol. Res., 1996, vol. 19, pp. 507-513.
14. Yun S.I., Cho H.R., Choi H.S. Anticoagulant from Taraxacum platycarpum. Biosci. Biotechnol. Biochem., 2014, vol. 66, pp. 1859-1864.
