Metabolic syndrome (MS) is a complex of metabolic, hormonal and hemodynamic disorders. MS contributes to the change in the structural and functional status of red blood cells and the dysregulation of their cation-transport function, in which Gardos channels - Ca2+-dependent potassium channels (KСа channels) play an important role. The MS was performed on Wistar male rats (23 animals), which were separated into the control and experimental groups. Rats from the control group were on a standard rat chow. The rats from the experimental group had for 12 weeks a high-fat, high-carbohydrate diet. The amplitude of the hyperpolarization response (HR) of erythrocytes was evaluated by the potentiometric method. It was shown that the amplitude of the HR of rat erythrocytes with MS changed depending on the acting agent: after stimulated with calcium ionophore A23187 - decreased, after the electron donor system was applied - increased compared to the amplitude of HR rats of the control group. It was found that in the isoosmotic incubation medium there was a decrease in the optical density of the erythrocyte suspension obtained from animals fed a high-fat, high-carbohydrate diet, but an increase in the optical density was observed when the rat erythrocyte suspension with MS was placed in a hyperosmotic medium. These data indicate that changes in the Ca2+ - dependent potassium permeability of the red blood cell membrane and erythrocyte volume in animals fed a high-fat, high-carbohydrate diet are associated with structural and physicochemical changes of the erythrocyte membrane.
erythrocytes, КСа channels, metabolic syndrome
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