In this work, we divided the absorption spectrum of the acetone extract of the Tetraselmis viridis microalgae by GPS into the spectra of individual pigments. The main pigments were lutein, neoxanthin chlorophyll a and chlorophyll b. In the culture spectrum, using the second-order derivative, individual absorption maxima were revealed. The data obtained are taken as a basis when compiling a model of the spectrum of native pigment forms. Models of native forms of chlorophyll a , chlorophyll b , and total photoprotective carotenoids are proposed. The obtained mathematical models of the absorption spectra of individual pigments and literature extinction coefficients can be used to quickly calculate the concentration of pigments in the culture.
marine microalgae, Tetraselmis viridis, chlorophyll a, chlorophyll b, absorption spectra, gaussians
1. Bidigare R.R. et al. In-vivo absorption properties of algal pigments. Ocean Optics X. International Society for Optics and Photonics, 1990, vol. 1302, pp. 290-302.
2. Churilova T.Ya., Finenko Z.Z., Akimov A.I. Mikrovodorosli Chernogo morya: problemy sohraneniya bioraznoobraziya i biotehnologicheskogo ispol'zovaniya. Sevastopol': EKOSI-Gidrofizika, 2008. @@[Churilova T.Ya., Finenko Z.Z., Akimov A.I. Black Sea microalgae: problems of biodiversity conservation and biotechnological use. Sevastopol: ECOSI-Hydrophysics, 2008. (In Russ.)]
3. Guiry M.D., Guiry G.M. 2019. AlgaeBase. World-wide electronic publication, National University of Ireland, Galway (taxonomic information republished from AlgaeBase with permission of M.D. Guiry). Tetraselmis viridis (Rouchijajnen) R.E.Norris, Hori & Chihara, 1980, URL: http://www.marinespecies.org/aphia.php?p=taxdetails& id=838159 on 2019-12-10.
4. Rouhiyaynen M.I. Novyy vid roda Platymonas (Chlorophyta) iz zelenyh vodorosley. Novosti sistematiki nizshih rasteniy. M.-L: Nauka, 1966. s. 82-85. @@[Rokhiyaynen M.I. A new species of the genus Platymonas (Chlorophyta) from green algae. News of the taxonomy of lower plants. M.-L: Nauka, 1966, pp. 82-85. (In Russ.)]
5. Trenkenshu R. P., Terskov I.A., Sid'ko F.Ya. Plotnye kul'tury morskih mikrovodorosley. Izv. SO AN SSSR (ser. Biol.), 1981, № 15, vyp. 3, s. 75-82. @@[Trenkenshu R.P., Terskov I.A., Sidko F.Ya. Dense cultures of marine microalgae. Izv. SB USSR Academy of Sciences (ser. Biol.), 1981, no. 15, iss. 3, pp. 75-82. (In Russ.)]
6. Trenkenshu R.P. Prosteyshie modeli rosta mikrovodorosley. 1. Periodicheskaya kul'tura. Ekologiya morya, 2005, vyp. 67, s. 89-97. @@[Trenkenshu R.P. The simplest models of microalgae growth. 1. Periodic culture // Ecology of the sea, 2005, iss. 67, pp. 89-97. (In Russ.)]
7. Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. Unificirovannaya ustanovka dlya laboratornyh issledovaniy mikrovodorosley. Voprosy sovremennoy al'gologii, 2017, № 1 (13). URL: http://algology.ru/1097 @@[Trenkenshu R.P., Lelekov A.S., Borovkov A.B., Novikova T.M. Unified installation for laboratory research of microalgae. Questions of modern algology, 2017, no. 1 (13). URL: http://algology.ru/1097. (In Russ.)]
8. Borovkov A.B., Gevorgiz R.G Produktivnost' Spirulina platensis i Tetraselmis viridis pri ispol'zovanii razlichnyh metodov kul'tivirovaniya. Ekologiya morya, 2005, vyp. 70, c. 9-13. @@[Borovkov A.B., Gevorgiz R.G. Productivity of Spirulina platensis and Tetraselmis viridis when using various cultivation methods. Ecology of the Sea, 2005, iss. 70, pp. 9-13. (In Russ.)]
9. Vladimirova M.G., Semenenko V.E. Intensivnaya kul'tura odnokletochnyh vodorosley. Moskva. AN SSSR, 1962, 60 s. @@[Vladimirova M.G., Semenenko V.E. Intensive culture of unicellular algae. Moscow. USSR Academy of Sciences, 1962, 60 p. (In Russ.)]
10. Kopytov Yu.P. i dr. Metodika kompleksnogo opredeleniya biohimicheskogo sostava mikrovodorosley. Al'gologiya, 2015. @@[Kopytov Yu.P. et al. Methodology for the complex determination of the biochemical composition of microalgae. Algology, 2015. (In Russ.)]
11. Wellburn R.W. The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Journal of plant physiology, 1994, vol. 144, no. 3, pp. 307-313.
12. Küpper H., Seibert S., Parameswaran A. Fast, sensitive, and inexpensive alternative to analytical pigment HPLC: quantification of chlorophylls and carotenoids in crude extracts by fitting with Gauss peak spectra. Analytical chemistry, 2007, vol. 79, no. 20, pp. 7611-7627.
13. Raes E.J., Isdepsky A, Muylaert K, Borowitzka M.A, Moheimani N.R. Comparison of growth of Tetraselmis in a tubular photobioreactor (Biocoil) and a raceway pond. J. Appl. Phycol., 2014, vol. 26, pp. 247-255, DOI:https://doi.org/10.1007/s10811-013-0077-5.
14. Gulyaev B.A., Litvin F.F. Pervaya i vtoraya proizvodnaya spektra pogloscheniya hlorofilla i soprovozhdayuschih pigmentov v kletkah vysshih rasteniy i vodorosley pri 20oS. Biofizika, 1970, t. 15, № 4, s. 670-680. @@[Gulyaev B.A., Litvin F.F. The first and second derivative of the absorption spectrum of chlorophyll and accompanying pigments in the cells of higher plants and algae at 20°C. Biophysics, 1970, vol. 15, no. 4, pp. 670-680. (In Russ.)]
15. Hoepffner N., Sathyendranath S. Effect of pigment composition on absorption properties of phytoplankton. Mar. Ecol. Prog. Ser., 1991, vol. 73, no. 1, pp. 11-23.
16. Smith E.L. The action of sodium dodecyl sulfate on the chlorophyll-protein compound of the spinach leaf. The Journal of General Physiology, 1941, vol. 24, no. 5, p. 583.
17. Mantoura R. F. C., Wright S. W. Phytoplankton pigments in oceanography: guidelines to modern methods. Oceanographic Literature Review, 1997, vol. 10, no. 44, p. 1110
