It was investigated the levels of nitric oxide, pro-inflammatory cytokines (IL-1β, IL-6, IL-18, TNFα), leptin and uric acid in blood plasma and synovial fluid (SF) as biomarkers of post-traumatic gonarthrosis (PTGA), as well as their association with radiological stage of PTGA. It was found that in patients with early and late stages of post-traumatic arthrosis of the knee joint, the level of circulating pro-inflammatory mediators: nitrites / nitrates (NOx), IL-6, and uric acid was increased compared with healthy individuals. Circulating levels of NOx, IL-1, IL-6, uric acid, leptin, and a combination of IL-6 and leptin can be potential diagnostic tests according to the ROC curve analysis. Plasma levels of NOx, IL-6, IL-18, and leptin significantly correlated with their levels in the SF. The severity of PTGA correlated with the level of leptin in plasma and SF, as well as with the level of synovial IL-18. The association of polymorphic variants of the nNOS and eNOS genes with the risk of developing post-traumatic gonarthrosis (PTGA) in residents of the Rostov region was studied. It has been established that the -84A allele of the nNOS polymorphic gene is associated with the risk of developing PTGA.
post-traumatic gonarthrosis, blood plasma, synovial fluid, nitric oxide, pro-inflammatory cytokines, leptin, NO synthase genes
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