Moscow State Academy of Veterinary Medicine and Biotechnology - MVA by K.I. Skryabin
Russian National Research and Technological Poultry Farming Institute, RAS
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
Moscow, Moscow, Russian Federation
By means of highly sensitive and specific enzyme sensor it was shown, that most of living tissues contain less than 50-100 nM of nitrite and nitrosamines and up to tens of μM of NO donors - S-nitrosothiols (RSNO), dinitrosyl iron complexes (DNIC) and high molecular weight nitrocompounds, that can turn to DNIC (RNO2). This fact means that living tissues can prevent NO oxidation to produce toxic compounds. NO donors - are stable compounds and they are not spontaneously decomposed with free NO release. The main pool of NO donors in most tissues is represented by DNIC. NO can transfer from the complex to the target at the moment of the complex destruction under the action of more effective iron chelators than the ligands of the DNIC. The transition is carried out with a minimum stay of NO in the free state. If the complex is exposed to an effective iron chelator, but there is no target, an iron-nitrosyl complex containing this chelator is formed. We assume, that some parts of the apoenzymes of NO targets can play the role of competitive chelators. Therefore, the physiological effect of NO donors depend not on their ability to produce free NO, but on the presence and characteristics of the physiological target. Not free NO accidentally finds the target, but the target, interacts with the NO donor causing its destruction and attaching NO. The effectiveness of DNIC as NO donor also depends on the DNIC structure and the ligand type. DNIC containing ligands with a high affinity for iron is more difficult to destroy by an iron chelator, which is necessary for the transfer of NO to the target. These effects were demonstrated both in model systems and living organisms.
nitric oxide, dinitrosyl iron complexes (DNIC), DNIC ligands
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