It is shown that the optical density (D750) of a suspension of Porphyridium purpureum and absolutely dry weight are linearly related. Using the method of least squares, the coefficient of connection between the optical density D750 and the wet weight of the culture was calculated, which is 0.051.Knowing the proportion of water in the wet mass of P. purpureum (0.132), it is possible to determine the relationship between the optical density and dry weight of P. purpureum , where the coefficient of relationship between the optical density of culture and dry weight is 0.395. It is noted that the determination of P. purpureum biomass by optical density of the culture on KFK-3 depends on the position of the cuvette in the cuvette holder and can change the instrument values by 1.9 times. Since when using KFK-3, the sum of the values of optical density and light scattering (attenuation) is measured. Thus, an express method for assessing the density of P. purpureum biomass by optical density has been developed.
methods for measuring density, optical density, red microalgae
1. Abdullaev A.A., Seminenko V.E. Intensivnaya kul'tura Dunaliella salina Teod i nekotorye ee fiziologicheskie harakteristiki. Fiziologiya rasteniy, 1974, t. 21, vyp. 6, s. 1145-1152. @@Abdullaev A.A., Seminenko V.E.Intensive culture of Dunaliella salina Teod and some of its physiological characteristics. Fiziologiya rasteniy, 1974, vol. 21, iss. 6, pp. 1145-1152. (In Russ.)
2. Gevorgiz R.G., Alisievich A.V., Shmatok M.G. Ocenka biomassy Spirulina platensis (Nordst.) Geitl po opticheskoy plotnosti kul'tury. Ekologiya morya, 2005, vyp. 70, s. 96-106. @@Gevorgiz R.G., Alisievich A.V., Shmatok M.G. Estimate of the biomass of Spirulina platensis (Nordst.) Geitl. by optical density of culture. Ekologiya moray, 2005, vol. 70, pp. 96-106. (In Russ.)
3. Gevorgiz R.G., Zheleznova S.N., Nikonova L.L., Bobko N.I., Nehoroshev M.V. Ocenka plotnosti kul'tury fototrofnyh mikroorganizmov metodom yodatnoy okislyaemosti. Sevastopol', 2015, 31 s. @@Gevorgiz R.G., Zheleznova S.N., Nikonova L.L., Bobko N.I., Nekhoroshev M.V. Evaluation of the culture density of phototrophic microorganisms by the method of iodate oxidizability. Sevastopol, 2015, 31 p. (In Russ.)
4. Wang S., Verma S.K., Said I.H., Thomsen L., Ullrich M.S., Kuhnert N. Changes in the fucoxanthin production and protein profiles in Cylindrotheca closterium in response to blue light-emitting diode light. Microbial Cell Factories, 2018, vol. 17, iss. 1, pp. 1-13. DOI:https://doi.org/10.1186/s12934-018-0957-0
5. Balti R., Le Balc’h R., Brodu N., Gilbert M., Le Gouic B., Le Gall S., Sinquin C., Masse A. Concentration and purification of Porphyridium cruentum exopolysaccharides by membrane filtration at various cross-flow velocities. Process Biochemistry, 2018, vol. 74, pp. 175-184. DOI:https://doi.org/10.1016/j.procbio.2018.06.021
6. Li T., Xu J., Wu H., Jiang P., Chen Z., Xiang W. Growth and biochemical composition of Porphyridium purpureum SCS-02 under different nitrogen concentrations. Marine Drugs, 2019, vol. 17, iss. 2, pp. 1-16.
7. Rodas-Zuluaga L.I., Castillo-Zacarías C., NúñezGoitia G., Martínez-Prado M.A., Rodríguez-Rodríguez J., LópezPacheco I.Y., Sosa-Hernández J.E., Iqbal H.M.N., Parra-Saldívar R. Implementation of kLa-Based strategy for Scaling Up Porphyridium purpureum (red marine microalga) to produce high-value phycoerythrin, fatty acids, and proteins. Marine Drugs, 2021, vol. 19, iss. 6, pp. 1-15. DOI:https://doi.org/10.3390/md19060290
8. Trenkenshu R.P., Terskov I.A., Sid'ko F.Ya. Plotnye kul'tury morskih mikrovodorosley. Izvestiya Sibirskogo otdeleniya Akademii nauk SSSR. Seriya biologicheskih nauk, 1981, t. 5, № 1, s. 75-82. @@Trenkenshu R.P., Terskov I.A., Sidko F.Ya. Dense cultures of marine microalgae. Bulletin of the Siberian Branch of the USSR Academy of Sciences. Biological Science Series, 1981, vol. 5, iss. 1, pp. 75-82. (In Russ.)
9. Lu L., Yang G., Zhu B., Pan K. A comparative study on three quantitating methods of microalgal biomass. Indian Journal of Geo-Marine Sciences, 2017, vol. 46, iss. 1, pp. 2265-2272.
10. Mel'nikov S.S., Samovich T.V., Manankina E.E., Budakova E.A. Vliyanie cheredovaniya svetovyh i temnovyh periodov na produktivnost' Spirulina (Arthrospira) platensis (Nordst.) Geitler. Al'gologiya, 2012, t. 22, № 2, s. 121-130. http://dspace.nbuv.gov.ua/handle/123456789/64212 @@Melnikov S.S., Samovich T.V., Manankina E.E., Budakova E.A. Influence of light and dark periods alternation on production of Spirulina (Arthrospira) platensis (Nordst.) Geitler. Algologiya, 2012, vol. 22, iss. 2, pp. 121-130. (In Russ.)
11. Sid'ko F.Ya., Eroshin N.S. Opredelenie koncentracii pigmentov i chisla kletok vo vzvesi vodorosley na fotoelektrokolorimetre FEKN-57. Upravlyaemoe kul'tivirovanie mikrovodorosley. M.: Nauka, 1964, c. 38-42. @@Sidko F.Ya., Eroshin N.S. Determination of the concentration of pigments and the number of cells in a suspension of algae using a FEKN-57 photoelectric colorimeter. Guided microalgae cultivation. Moscow: Nauka, 1964, pp. 38-42. (In Russ.)
12. Sid'ko F.Ya., Eroshin N.S., Belyanin V.N. Datchik opticheskoy plotnosti, izgotovlennyy na baze fotoelektrokolorimetra FEKM. Nepreryvnoe upravlyaemoe kul'tivirovanie mikroorganizmov. Moskva: Nauka, 1967, s. 31-33. @@Sidko F.Ya., Eroshin N.S., Belyanin V.N. Optical density sensor based on the FEKM photoelectric colorimeter. Continuous controlled cultivation of microorganisms. Moscow: Nauka, 1967, pp. 31-33. (In Russ.)